Hollow sperm syndrome during spermatogenesis in the giant tiger shrimp Penaeus monodon (Fabricius 1798) from eastern Australia

• Published in: Aquaculture research Vol. 46; no. 11; pp. 2573 - 2592
• Main Authors: Chong, Roger S‐M, Cowley, Jeff A, Paterson, Brian D, Coman, Greg J, Mann, David L, Arnold, Stuart J, Prior, Howard C, Wood, Andrew T, Amigh, Mohammed J
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Science 01.11.2015; Blackwell Publishing Ltd; Hindawi Limited
• Subjects: adults; breeding stock; chromatin; farms; hollow sperm; male fertility; Penaeus monodon; ponds; shrimp; sperm abnormalities; spermatids; spermatogenesis; spermatophores; spermatozoa; stocking rate; tanks; temperature; testes; transmission electron microscopy; Queensland
• ISSN: 1355-557X; 1365-2109
• DOI: 10.1111/are.12411

During spermatogenesis, giant tiger shrimp (Penaeus monodon) from Queensland, eastern Australia had a high proportion of testicular spermatids that appeared ‘hollow’ because their nuclei were not visible with the haematoxylin and eosin stain. When examined by transmission electron microscopy, the nuclei of hollow spermatids contained highly decondensed chromatin, with large areas missing fibrillar chromatin. Together with hollow spermatids, testicular pale enlarged (PE) spermatids with weakly staining and marginated chromatin were observed. Degenerate‐eosinophilic‐clumped (DEC) spermatids that appeared as aggregated clumps were also present in testes tubules. Among 171 sub‐adult and adult P. monodon examined from several origins, 43% displayed evidence of hollow spermatids in the testes, 33% displayed PE spermatids and 15% displayed DEC spermatids. These abnormal sperm were also found at lower prevalence in the vas deferens and spermatophore. We propose ‘Hollow Sperm Syndrome (HSS)’ to describe this abnormal sperm condition as these morphological aberrations have yet to be described in penaeid shrimp. No specific cause of HSS was confirmed by examining either tank or pond cultured shrimp exposed to various stocking densities, temperatures, salinities, dietary and seasonal factors. Compared with wild broodstock, HSS occurred at higher prevalence and severity among sub‐adults originating from farms, research ponds and tanks. Further studies are required to establish what physiological, hormonal or metabolic processes may cause HSS and whether it compromises the fertility of male P. monodon.