Inducible and reversible RNA N6-methyladenosine editing

• Published in: Nature communications Vol. 13; no. 1; p. 1958
• Main Authors: Shi, Huaxia, Xu, Ying, Tian, Na, Yang, Ming, Liang, Fu-Sen
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.04.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38/109; 38/71; 38/88; 42/41; 49/90; 631/337/4041/3196; 631/61/212/177; 631/92/458/1648; Abscisic acid; CRISPR; Editing; Epigenetics; Humanities and Social Sciences; multidisciplinary; N6-methyladenosine; Ribonucleic acid; RNA; RNA editing; RNA modification; Science; Science (multidisciplinary); Transcription
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-29665-y

RNA modifications, including N 6 -methyladenosine (m 6 A), have been reported to regulate fundamental RNA processes and properties, and directly linked to various human diseases. Methods enabling temporal and transcript/locus-specific editing of specific RNA modifications are essential, but still limited, to dissect the dynamic and context-dependent functions of these epigenetic modifications. Here, we develop a chemically inducible and reversible RNA m 6 A modification editing platform integrating chemically induced proximity (CIP) and CRISPR methods. We show that m 6 A editing can be temporally controlled at specific sites of individual RNA transcripts by the addition or removal of the CIP inducer, abscisic acid (ABA), in the system. By incorporating a photo-caged ABA, a light-controlled version of m 6 A editing platform can be developed. We expect that this platform and strategy can be generally applied to edit other RNA modifications in addition to m 6 A. RNA modifications, including N6-methyladenosine (m6A), have been reported to regulate fundamental RNA processes and properties, and directly linked to various human diseases. Here, the authors develop a chemically inducible and reversible RNA m6A modification editing platform integrating chemically induced proximity (CIP) and CRISPR methods.