Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

• Published in: RSC advances Vol. 12; no. 8; pp. 4697 - 472
• Main Authors: Gao, Mochou, Liu, Qin, Xue, Yuanyuan, Li, Bao, Liu, Xingchi, Shi, Zhenzhu, Liu, Nan, Zou, Xueyan
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 03.02.2022; The Royal Society of Chemistry
• Subjects: Adsorbents; Affinity; Chemistry; Diameters; Glutathione; Nanospheres; Peanuts; Pore size; Proteins; Separation; Silicon dioxide; Tin
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d1ra08362g

A peanut-like hollow silica (denoted as p-l-hSiO 2 ) adsorbent is prepared in a facile method, which is composed of several silica nanospheres and has an average diameter of 22 nm, with thickness of 5 nm. Its Brunauer-Emmett-Teller (BET) surface area, pore volume and pore size are 258.9 m 2 g −1 , 1.56 cm 3 g −1 and 3.9 nm, respectively. Then the afforded p-l-hSiO 2 /GSH adsorbent is applied to purify glutathione S-transferases-tagged (denoted as GST-tagged) proteins. It is found that the p-l-hSiO 2 adsorbent exhibits a specific adsorption, a high binding capacity (6.80 mg g −1 ), good recycling performance and high recovery (90.1%) to the target proteins, showing promising potential for the affinity separation of GST-tagged proteins. Peanut-like Sn-doped hollow silica adsorbent is prepared in a facile method, which exhibits a specific adsorption, a high binding capacity , good recycle performance and high recovery to the GST-tagged proteins.