Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43

Virion RNAs from the bovine enteric coronavirus and the human respiratory coronavirus OC43 were compared by one dimensional gel electrophoresis and by oligonucleotide fingerprinting. For each virus, approximately 55 per cent of the RNA migrated as a 6.8 Md species, 10 per cent as a 0.68 Md species,...

Full description

Saved in:
Bibliographic Details
Published inArchives of virology Vol. 86; no. 1/2; pp. 101 - 108
Main Authors Lapps, W, Brian, D.A
Format Journal Article
LanguageEnglish
Published Austria 01.01.1985
Subjects
Animals
antigens
Base Sequence
Cattle
Cell Line
Coronaviridae
Coronaviridae - genetics
Genes, Viral
Humans
RNA, Viral - analysis
Online AccessGet more information

Cover

More Information
Summary:Virion RNAs from the bovine enteric coronavirus and the human respiratory coronavirus OC43 were compared by one dimensional gel electrophoresis and by oligonucleotide fingerprinting. For each virus, approximately 55 per cent of the RNA migrated as a 6.8 Md species, 10 per cent as a 0.68 Md species, and 15 per cent as heterogeneous small molecular weight RNA. A sequence homology of greater than 96 per cent was observed between the 6.8 Md species from the two viruses. The 0.68 Md RNA is apparently an intravirion, subgenomic, polyadenylated molecule based on RNAse studies, oligo (dT)-cellulose chromatography, and hybridization to a cDNA clone of the 3' terminal 1.19 Kb region of the bovine coronavirus genome.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01314116