Modeling rapid and selective capture of nNOS-PSD-95 uncouplers from Sanhuang Xiexin decoction by novel molecularly imprinted polymers based on metal-organic frameworks
Novel and highly selective molecularly imprinted polymers based on the surface of metal-organic frameworks, NH 2 -MIL-101(Cr) (MIL@MIP S ), were successfully fabricated to capture neuronal nitric oxide synthase-postsynaptic density protein-95 (nNOS-PSD-95) uncouplers from Sanhuang Xiexin Decoction (...
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Published in | RSC advances Vol. 1; no. 13; pp. 7671 - 7681 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
21.02.2020
The Royal Society of Chemistry |
Subjects | |
Online Access | Get full text |
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Summary: | Novel and highly selective molecularly imprinted polymers based on the surface of metal-organic frameworks, NH
2
-MIL-101(Cr) (MIL@MIP
S
), were successfully fabricated to capture neuronal nitric oxide synthase-postsynaptic density protein-95 (nNOS-PSD-95) uncouplers from Sanhuang Xiexin Decoction (SXD) for stroke treatment. The resultant polymers were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and X-ray diffraction. The performance tests revealed that MIL@MIPs had a large binding capacity, fast kinetics, and excellent selectivity. Then the obtained polymers were satisfactorily applied to solid-phase extraction coupled with high-performance liquid chromatography to selectively capture nNOS-PSD-95 uncouplers from SXD. Furthermore, the biological activities of components obtained from SXD were evaluated
in vivo
and
in vitro
. As a consequence, the components showed a potent neuroprotective effect from the MTS assay and uncoupling activity from the co-immunoprecipitation experiment. In addition, the anti-ischemic stroke assay
in vivo
was further investigated to determine the effect of reducing infarct size and ameliorating neurological deficit by the active components. Therefore, this present study contributes a valuable new method and new tendency to selectively capture active components for stroke treatment from SXD and other natural medicines.
Novel MIL@MIPs were prepared to rapidly capture nNOS-PSD-95 uncouplers from Sanhuang Xiexin decoction, coupled with SPE and HPLC. |
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Bibliography: | 10.1039/c9ra10537a Electronic supplementary information (ESI) available. See DOI ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2046-2069 2046-2069 |
DOI: | 10.1039/c9ra10537a |