Putrescine-polysaccharide conjugates as transglutaminase substrates and their possible use in producing crosslinked films

• Published in: Amino acids Vol. 38; no. 2; pp. 669 - 675
• Main Authors: Di Pierro, Prospero, Mariniello, Loredana, Sorrentino, Angela, Villalonga, Reynaldo, Chico, Belkis, Porta, Raffaele
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.02.2010; Springer Vienna; Springer Nature B.V
• Subjects: Alginates - chemistry; Analytical Chemistry; Bacterial Proteins - chemistry; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Biotechnology; Conjugates; Covalence; Cross-Linking Reagents - chemistry; Crosslinking; Flour; Food science; Glucuronic Acid - chemistry; Hexuronic Acids - chemistry; Life Sciences; Microorganisms; Neurobiology; Original Article; Pectin; Pectins - chemistry; Polyamines; Polysaccharides; Polysaccharides - chemical synthesis; Polysaccharides - chemistry; Protein Binding; Proteins; Proteomics; Putrescine - chemistry; Soybean Proteins - chemistry; Streptomyces - enzymology; Substrate Specificity; Transglutaminases - chemistry; Transglutaminase; Pectin; Alginate; Putrescine; Crosslink; Edible films
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-009-0427-4

Putrescine (1,4-diaminobutane) was covalently linked to alginate and low-methoxyl pectin to synthesize new aminated polysaccharides. Both putrescine-pectin and -alginate conjugates, although the latter at higher concentrations, were found to be able to act as effective acyl acceptor transglutaminase substrates in vitro using both dimethylated casein and soy flour proteins as acyl donors. Monodansylcadaverine, a well known acyl acceptor transglutaminase substrate, dose-dependently counteracted the covalent binding of the aminated polysaccharides to the proteins. Putrescine-pectin conjugate was also tested to prepare, in combination with soy flour proteins, edible films in the presence of purified microbial transglutaminase. Characterization of the enzymatically crosslinked films showed a significant decreased water vapor permeability, with respect to the ones obtained with non-aminated pectin in the presence of transglutaminase, as well as improved mechanical properties, such as high extensibility. Possible biotechnological applications of hydrocolloid films containing putrescine-polysaccharide derivatives enzymatically crosslinked to proteins were suggested.