A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

Highlights • We developed a novel identification method for vaginal secretions. • This method is based on relative quantification of vaginal Lactobacillus species. • The measured ΔCt values were significantly low in vaginal secretions. • This ΔCt method is simple and sensitive. • The ΔCt value can b...

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Published inForensic science international : genetics Vol. 12; pp. 93 - 99
Main Authors Doi, Masanori, Gamo, Shinsuke, Okiura, Tatsuyuki, Nishimukai, Hiroaki, Asano, Migiwa
Format Journal Article
LanguageEnglish
Published Netherlands 01.09.2014
Subjects
Base Sequence
DNA Primers
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Female
Humans
Lactobacillus - genetics
Pathology
Real-Time Polymerase Chain Reaction
RNA, Ribosomal, 16S - genetics
Vagina - microbiology
Vagina - secretion
Vaginal secretions
Microflora
Real time PCR
Body fluid identification
Human DNA
Lactobacillus
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Summary:Highlights • We developed a novel identification method for vaginal secretions. • This method is based on relative quantification of vaginal Lactobacillus species. • The measured ΔCt values were significantly low in vaginal secretions. • This ΔCt method is simple and sensitive. • The ΔCt value can be used as an indicator for the presence of vaginal secretions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2014.05.005