Wolbachia infection and cytoplasmic incompatibility in the cricket Teleogryllus taiwanemma

• Published in: Journal of experimental biology Vol. 203; no. Pt 16; pp. 2503 - 2509
• Main Authors: Kamoda, S, Masui, S, Ishikawa, H, Sasaki, T
• Format: Journal Article
• Language: English
• Published: England 15.08.2000
• Subjects: Animals; Bacterial Outer Membrane Proteins - genetics; Cytoplasm - microbiology; Female; Genes, Bacterial; Gryllidae; Gryllidae - microbiology; Gryllidae - physiology; Male; Phylogeny; Proteins - isolation & purification; Reproduction; Spermatozoa - chemistry; Symbiosis; Teleogryllus taiwanemma; Wolbachia; Wolbachia - genetics; Wolbachia - physiology
• ISSN: 0022-0949; 1477-9145
• DOI: 10.1242/jeb.203.16.2503

Wolbachia are cytoplasmically inherited bacteria found in many arthropods. They induce various reproductive alterations in their hosts, including cytoplasmic incompatibility, thelytokous parthenogenesis, feminization and male-killing. In this study, we examined Wolbachia infection and its effects on the host cricket Teleogryllus taiwanemma. In a phylogenetic study based on the wsp gene coding for a Wolbachia surface protein, the Wolbachia strain harboured by T. taiwanemma was clustered together with those harboured by Laodelphax striatellus, Tribolium confusum, Acraea encedon, Trichogramma deion and Adalia bipunctata. Crossing experiments using the Wolbachia-infected and uninfected strains of cricket showed that the infection is associated with the expression of unidirectional cytoplasmic incompatibility: the egg hatch rate in the incompatible cross between the infected males and uninfected females was 20.3 %. We also examined the distribution of Wolbachia within the host using polymerase chain reaction assays; they were detected in the antennae, heads, forewings, hindwings, testes, ovaries, Malpighian tubules, foot muscles and fat bodies. Quantitative polymerase chain reaction assays showed that the bacterial density was highest in the fat bodies, followed by the ovaries and testes. Wolbachia were not detected in the haemolymph or in mature spermatozoa. The spermatozoa of the infected male may be modified by the presence of Wolbachia during its development. To examine this possibility, we compared the profiles of sperm proteins between the infected and uninfected males using two-dimensional gel electrophoresis. However, no differences in the protein profiles were observed.