Therapeutic HNF4A mRNA attenuates liver fibrosis in a preclinical model

• Published in: Journal of hepatology Vol. 75; no. 6; pp. 1420 - 1433
• Main Authors: Yang, Taihua, Poenisch, Marion, Khanal, Rajendra, Hu, Qingluan, Dai, Zhen, Li, Ruomeng, Song, Guangqi, Yuan, Qinggong, Yao, Qunyan, Shen, Xizhong, Taubert, Richard, Engel, Bastian, Jaeckel, Elmar, Vogel, Arndt, Falk, Christine S., Schambach, Axel, Gerovska, Daniela, Araúzo-Bravo, Marcos J., Vondran, Florian W.R., Cantz, Tobias, Horscroft, Nigel, Balakrishnan, Asha, Chevessier, Frédéric, Ott, Michael, Sharma, Amar Deep
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021; Elsevier Science Ltd
• Subjects: Animal models; Animals; Biodegradability; Cholestasis; Chromatin; Cirrhosis; Disease Models, Animal; DNA microarrays; Fibrosis; Gene expression; Hepatocyte nuclear factor 4; Hepatocyte Nuclear Factor 4 - pharmacology; Hepatocyte Nuclear Factor 4 - therapeutic use; Hepatocytes; Hepatotoxicity; Immunoprecipitation; Liver; Liver cirrhosis; Liver Cirrhosis - drug therapy; Macrophages; Mice; mRNA therapeutics; Nanoparticles; Paraoxonase; Paraoxonase 1; protein replacement and cirrhosis; RNA, Messenger - pharmacology; RNA, Messenger - therapeutic use; Stellate cells; Therapeutic targets; Transcription factors; Transfection; mRNA therapeutics; Transcription factors; protein replacement and cirrhosis
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/j.jhep.2021.08.011

Therapeutic targeting of injuries that require transient restoration of proteins by mRNA delivery is an attractive approach that, until recently, has remained poorly explored. In this study, we examined the therapeutic utility of mRNA delivery for liver fibrosis and cirrhosis. Specifically, we aimed to demonstrate the therapeutic efficacy of human hepatocyte nuclear factor alpha (HNF4A) mRNA in mouse models of fibrosis and cirrhosis. We investigated restoration of hepatocyte functions by HNF4A mRNA transfection in vitro, and analyzed the attenuation of liver fibrosis and cirrhosis in multiple mouse models, by delivering hepatocyte-targeted biodegradable lipid nanoparticles (LNPs) encapsulating HNF4A mRNA. To identify potential mechanisms of action, we performed microarray-based gene expression profiling, single-cell RNA sequencing, and chromatin immunoprecipitation. We used primary liver cells and human liver buds for additional functional validation. Expression of HNF4A mRNA led to restoration of the metabolic activity of fibrotic primary murine and human hepatocytes in vitro. Repeated in vivo delivery of LNP-encapsulated HNF4A mRNA induced a robust inhibition of fibrogenesis in 4 independent mouse models of hepatotoxin- and cholestasis-induced liver fibrosis. Mechanistically, we discovered that paraoxonase 1 is a direct target of HNF4A and it contributes to HNF4A-mediated attenuation of liver fibrosis via modulation of liver macrophages and hepatic stellate cells. Collectively, our findings provide the first direct preclinical evidence of the applicability of HNF4A mRNA therapeutics for the treatment of fibrosis in the liver. Liver fibrosis and cirrhosis remain unmet medical needs and contribute to high mortality worldwide. Herein, we take advantage of a promising therapeutic approach to treat liver fibrosis and cirrhosis. We demonstrate that restoration of a key gene, HNF4A, via mRNA encapsulated in lipid nanoparticles decreased injury in multiple mouse models of fibrosis and cirrhosis. Our study provides proof-of-concept that mRNA therapy is a promising strategy for reversing liver fibrosis and cirrhosis. [Display omitted] •Restoration of HNF4A via mRNA delivery improves functions of fibrotic primary hepatocytes from both mice and humans.•The mRNA encapsulated in lipid nanoparticles can be delivered into hepatocytes of the fibrotic liver.•Lipid nanoparticle-mediated HNF4A mRNA delivery ameliorates fibrosis and cirrhosis in different chronic liver injury models.•Paraoxonase 1, a therapeutic target of HNF4A, contributes to anti-fibrotic effects of HNF4A.•HNF4A mRNA delivery affects macrophage infiltration and polarization as well as hepatic stellate cell activation.