Genome diversity in the genera Fructobacillus, Leuconostoc and Weissella determined by physical and genetic mapping

• Published in: Microbiology (Society for General Microbiology) Vol. 156; no. 2; pp. 420 - 430
• Main Authors: Chelo, Ivo M, Ze-Ze, Libia, Tenreiro, Rogerio
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.02.2010; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Chromosome Mapping; Chromosomes, Bacterial; DNA Restriction Enzymes - metabolism; Electrophoresis, Gel, Pulsed-Field; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genetics; Genome, Bacterial; Gram-Positive Bacteria - genetics; Leuconostoc; Leuconostoc - genetics; Leuconostocaceae - genetics; Microbiology; Physical Chromosome Mapping; Species Specificity; Weissella; Genetic mapping; Streptococcaceae; Lactic acid bacteria; Bacteria; Micrococcales; Genotype; Genetics; Leuconostoc; Genome; Weissella
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/mic.0.028308-0

Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências da Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal Pulsed-field gel electrophoresis analysis of chromosomal single and double restriction profiles of 17 strains belonging to three genera of ‘ Leuconostocaceae ’ was done, resulting in physical and genetic maps for three Fructobacillus , six Leuconostoc and four Weissella strains. Asc I, I- Ceu I, Not I and Sfi I restriction enzymes were used together with Southern hybridization of selected probes to provide an assessment of genomic organization in different species. Estimated genome sizes varied from 1408 kb to 1547 kb in Fructobacillus , from 1644 kb to 2133 kb in Leuconostoc and from 1371 kb to 2197 kb in Weissella . Other genomic characteristics of interest were analysed, such as oriC and terC localization and rrn operon organization. The latter seems markedly different in Weissella , in both number and disposition in the chromosome. Comparisons of intra- and intergeneric features are discussed in the light of chromosome rearrangements and genomic evolution. Correspondence Ivo M. Chelo imchelo{at}igc.gulbenkian.pt Abbreviations: LAB, lactic acid bacteria Present address: Instituto Gulbenkian de Ciência, Apartado 14, P-2781-901 Oeiras, Portugal. Present address: Centro de Estudos de Vectores e Doenças Infecciosas Dr Francisco Cambournac, Instituto Nacional de Saúde Dr Ricardo Jorge I.P., Av. Liberdade no. 5, 2965-575 Águas de Moura, Portugal. Supplementary material is available with the online version of this paper.