Reengineering of cancer cell surface charges can modulate cell migration

The ability to modulate the cell surface structure provides a powerful tool to understand fundamental processes and also to elicit desired cellular responses. Here we report the development of a new class of 'clickable labels' to reengineer the cell surface charges of live cells. The metho...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 58; no. 36; pp. 5522 - 5525
Main Authors Ghirardello, Mattia, Shyam, Radhe, Galan, M. Carmen
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 03.05.2022
Royal Society of Chemistry
The Royal Society of Chemistry
Subjects
Cancer
Cell adhesion & migration
Cell Membrane - metabolism
Cell Movement
Chemistry
Chemistry, Multidisciplinary
Humans
Labels
Neoplasms
Oligosaccharides
Oligosaccharides - metabolism
Physical Sciences
Reengineering
Science & Technology
Sialic Acids
Surface structure
ADHESION
ASSAY
ROLES
SIGLECS
GLYCOSYLATION
SIALIC ACIDS
PROGRESSION
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Summary:The ability to modulate the cell surface structure provides a powerful tool to understand fundamental processes and also to elicit desired cellular responses. Here we report the development of a new class of 'clickable labels' to reengineer the cell surface charges of live cells. The method relies on the use of metabolic oligosaccharide engineering (MOE) combined with chemo selective labeling of cell surface azido-containing sialic acids with dibenzocyclooctyne (DBCO) ionic-probes. Using this strategy, we demonstrate that reducing the negative charge induced by the overexpression of cell surface sialic acids in cancer cells leads to a reduction in cell migration without affecting drug supceptibility. Reducing the negative charges induced by the overexpression of cell surface sialic acids using cationic clickable labels leads to a reduction in cancer cell migration without affecting drug supceptibility.
Bibliography:https://doi.org/10.1039/d2cc00402j
Electronic supplementary information (ESI) available. See DOI
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These authors contributed equally to this work.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc00402j