Identification of Differentially Expressed Genes from Ovarian Cancer Cells by MICROMAX™ cDNA Microarray System

Using the MICROMAX™ cDNA microarray system, we were able to identify genes that are differentially overexpressed in ovarian cancer. A total of 30 putative genes, which are differentially overexpressed in ovarian cancer cell lines, were identified. The differential expression of some of these genes w...

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Bibliographic Details
Published inBioTechniques Vol. 30; no. 3; pp. 670 - 675
Main Authors Wong, Kwong-Kwok, Cheng, Rita S, Mok, Samuel C
Format Journal Article
LanguageEnglish
Published England Future Science Ltd 01.03.2001
Subjects
Female
Humans
Oligonucleotide Array Sequence Analysis
Ovarian Neoplasms - genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
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Summary:Using the MICROMAX™ cDNA microarray system, we were able to identify genes that are differentially overexpressed in ovarian cancer. A total of 30 putative genes, which are differentially overexpressed in ovarian cancer cell lines, were identified. The differential expression of some of these genes was further confirmed by real-time RT-PCR. Using this strategy, we have identified genes that either overexpress in all cancer cell lines or in only some cancer cell lines. Further characterization of these genes will allow them to be exploited in diagnosis, prognosis, anticancer therapy, and molecular classification of ovarian cancer.
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ISSN:0736-6205
1940-9818
DOI:10.2144/01303dd05