Misexpression of Cwnt8C in the mouse induces an ectopic embryonic axis and causes a truncation of the anterior neuroectoderm

• Published in: Development (Cambridge) Vol. 124; no. 15; pp. 2997 - 3005
• Main Authors: Pöpperl, H, Schmidt, C, Wilson, V, Hume, C R, Dodd, J, Krumlauf, R, Beddington, R S
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 01.08.1997
• Subjects: Actins - genetics; Animals; Basic Helix-Loop-Helix Transcription Factors; Body Patterning - genetics; Chickens; Cytoskeletal Proteins; Ectoderm - physiology; Gastrula; Gene Expression; Homeodomain Proteins - genetics; Humans; Mice; Mice, Transgenic; Promoter Regions, Genetic - genetics; Prosencephalon - embryology; Proteins - genetics; Proteins - physiology; Repressor Proteins; RNA, Messenger - analysis; Transcription Factor HES-1; Wnt Proteins; Zebrafish Proteins
• ISSN: 0950-1991; 1477-9129
• DOI: 10.1242/dev.124.15.2997

Transgenic embryos expressing Cwnt8C under the control of the human beta-actin promoter exhibit duplicated axes or a severely dorsalised phenotype. Although the transgene was introduced into fertilised eggs all duplications occurred within a single amnion and, therefore, arose from the production of more than one primitive streak at the time of gastrulation. Morphological examination and the expression of diagnostic markers in transgenic embryos suggested that ectopic Cwnt8C expression produced only incomplete axis duplication: axes were always fused anteriorly, there was a reduction in tissue rostral to the anterior limit of the notochord, and no duplicated expression domain of the forebrain marker Hesx1 was observed. Anterior truncations were evident in dorsalised transgenic embryos containing a single axis. These results are discussed in the light of the effects of ectopic Xwnt8 in Xenopus embryos, where its early expression leads to complete axis duplication but expression after the mid-blastula transition causes anterior truncation. It is proposed that while ectopic Cwnt8C in the mouse embryo can duplicate the primitive streak and node this only produces incomplete axis duplication because specification of the anterior aspect of the axis, as opposed to maintenance of anterior character, is established by interaction with anterior primitive endoderm rather than primitive streak derivatives.