Binding of Acanthamoeba to hydrogel contact lenses

The numbers of Acanthamoeba binding to new hydrogel contact lenses of different polymer and water content were determined with two quantitative methods, a radiolabeled-cell method and a detaching-fluid method. Numbers of amoebae retained on nonionic lenses increased with increasing water content of...

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Bibliographic Details
Published inCurrent eye research Vol. 15; no. 2; p. 151
Main Authors Gorlin, A I, Gabriel, M M, Wilson, L A, Ahearn, D G
Format Journal Article
LanguageEnglish
Published England 01.02.1996
Subjects
Acanthamoeba
Animals
Biofilms
Contact Lens Solutions
Contact Lenses
Equipment Contamination
Hydrogel, Polyethylene Glycol Dimethacrylate
Immersion
Polyethylene Glycols - chemistry
Water - analysis
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Summary:The numbers of Acanthamoeba binding to new hydrogel contact lenses of different polymer and water content were determined with two quantitative methods, a radiolabeled-cell method and a detaching-fluid method. Numbers of amoebae retained on nonionic lenses increased with increasing water content of the lenses. With both nonionic and ionic lenses numbers of associated amoebae decreased with successive rinsing steps. The retentions of amoebae on unworn hydrogel lenses, in contrast to the irreversible adhesion of bacteria, were tenuous and appeared to be effected mainly by surface tension, surface charge and water content.
ISSN:0271-3683
DOI:10.3109/02713689608997408