Longitudinal correlations between MRE, MRI-PDFF, and liver histology in patients with non-alcoholic steatohepatitis: Analysis of data from a phase II trial of selonsertib

• Published in: Journal of hepatology Vol. 70; no. 1; pp. 133 - 141
• Main Authors: Jayakumar, Saumya, Middleton, Michael S., Lawitz, Eric J., Mantry, Parvez S., Caldwell, Stephen H., Arnold, Hays, Mae Diehl, Anna, Ghalib, Reem, Elkhashab, Magdy, Abdelmalek, Manal F., Kowdley, Kris V., Stephen Djedjos, C., Xu, Ren, Han, Ling, Mani Subramanian, G., Myers, Robert P., Goodman, Zachary D., Afdhal, Nezam H., Charlton, Michael R., Sirlin, Claude B., Loomba, Rohit
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2019; Elsevier Science Ltd
• Subjects: Adolescent; Adult; Aged; Benzamides - administration & dosage; Biopsy; Data processing; Disease Progression; Dose-Response Relationship, Drug; Elasticity Imaging Techniques - methods; Fatty liver; Female; Fibrosis; Hepatic steatosis; Histology; Humans; Imidazoles - administration & dosage; Injections, Subcutaneous; Liver; Liver - pathology; Liver biopsy; Liver diseases; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Male; Middle Aged; NMR; Non-alcoholic fatty liver disease; Non-alcoholic Fatty Liver Disease - diagnosis; Non-alcoholic Fatty Liver Disease - drug therapy; Non-alcoholic steatohepatitis; Non-invasive tests; Nuclear magnetic resonance; Patients; Pyridines - administration & dosage; Reproducibility of Results; ROC Curve; Steatosis; Treatment Outcome; Young Adult; Liver biopsy; Non-invasive tests; Hepatic steatosis; Non-alcoholic fatty liver disease; Non-alcoholic steatohepatitis
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/j.jhep.2018.09.024

[Display omitted] •Liver stiffness by magnetic resonance elastography (MRE) correlated with fibrosis.•Reductions in liver stiffness by MRE were associated with improvement of fibrosis.•Assessment of liver fat by magnetic resonance imaging (MRI) correlated with severity of liver steatosis.•Reductions in MRI estimates of liver fat were correlated with reductions in liver fat on biopsy. Non-invasive tools for monitoring treatment response and disease progression in non-alcoholic steatohepatitis (NASH) are needed. Our objective was to evaluate the utility of magnetic resonance (MR)-based hepatic imaging measures for the assessment of liver histology in patients with NASH. We analyzed data from patients with NASH and stage 2 or 3 fibrosis enrolled in a phase II study of selonsertib. Pre- and post-treatment assessments included centrally read MR elastography (MRE)-estimated liver stiffness, MR imaging-estimated proton density fat fraction (MRI-PDFF), and liver biopsies evaluated according to the NASH Clinical Research Network classification and the non-alcoholic fatty liver disease activity score (NAS). Among 54 patients with MRE and biopsies at baseline and week 24, 18 (33%) had fibrosis improvement (≥1-stage reduction) after undergoing 24 weeks of treatment with the study drug. The area under the receiver operating characteristic curve (AUROC) of MRE-stiffness to predict fibrosis improvement was 0.62 (95% CI 0.46–0.78) and the optimal threshold was a ≥0% relative reduction. At this threshold, MRE had 67% sensitivity, 64% specificity, 48% positive predictive value, 79% negative predictive value. Among 65 patients with MRI-PDFF and biopsies at baseline and week 24, a ≥1-grade reduction in steatosis was observed in 18 (28%). The AUROC of MRI-PDFF to predict steatosis response was 0.70 (95% CI 0.57–0.83) and the optimal threshold was a ≥0% relative reduction. At this threshold, MRI-PDFF had 89% sensitivity and 47% specificity, 39% positive predictive value, and 92% negative predictive value. These preliminary data support the further evaluation of MRE-stiffness and MRI-PDFF for the longitudinal assessment of histologic response in patients with NASH. Liver biopsy is a potentially painful and risky method to assess damage to the liver due to non-alcoholic steatohepatitis (NASH). We analyzed data from a clinical trial to determine if 2 methods of magnetic resonance imaging – 1 to measure liver fat and 1 to measure liver fibrosis (scarring) – could potentially replace liver biopsy in evaluating NASH-related liver injury. Both imaging methods were correlated with biopsy in showing the effects of NASH on the liver.