The development of marine biomaterial derived from decellularized squid mantle for potential application as tissue engineered urinary conduit

• Published in: Materials Science & Engineering C Vol. 119; p. 111579
• Main Authors: Adamowicz, J., Kloskowski, T., Stopel, M., Gniadek, M., Rasmus, M., Balcerczyk, D., Buhl, M., Gagat, M., Antosik, P., Grzanka, D., Sionkowska, A., Drewa, T., Pokrywczynska, M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2021; Elsevier BV
• Subjects: Animals; Biocompatible Materials; Biomaterials; Biomechanical engineering; Biomechanics; Biomedical materials; Bladder; Computer applications; Decapodiformes; Decellularized biomaterial; Extracellular Matrix; Finite element method; Marine biomaterial; Materials science; Muscles; Polysaccharides; Reconstructive urology; Smooth muscle; Squid; Swine; Tissue Engineering; Tissue Scaffolds; Urinary Bladder; Urinary conduit; Urology; Urothelium; Tissue engineering; Marine biomaterial; Reconstructive urology; Decellularized biomaterial; Urinary conduit
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111579

Tissue engineering is focusing research effort on search for new biomaterials that might be applied to create artificial urinary conduit. Nevertheless, the demanding biomechanical characteristics necessary for proper conduit function is difficult to be replicated. In this study, we are introducing novel marine biomaterial obtained by decellularization of squid mantle derived from Loligo vulgaris. Squid mantles underwent decellularization according to developed dynamic flow two-staged procedure. Efficacy of the method was confirmed by computational dynamic flow analysis. Subsequently Decellularized Squid Mantle (DSM) underwent extensive histological analysis and mechanical evaluation. Based on gained biomechanical data the computational modelling using finite element method was utilized to simulate behavior of DSM used as a urinary conduit. Taking into account potential application in reconstructive urology, the DSM was then evaluated as a scaffold for urothelial and smooth muscle cells derived from porcine urinary bladder. Conducted analysis showed that DSM created favorable environment for cells growth. In addition, due to polarized structure and natural external polysaccharide layer, it protected seeded cells from urine. •Two-staged dynamic flow decellularization method was effective approach to obtain DSM.•Efficiency of decellularization method was confirmed with dynamic flow analysis.•DSM turned out to be suitable biomaterial for artificial urinary conduit.•Computational analysis was applied to evaluate DSM behavior as urinary conduit.•SMS and UC grew on DSM.