On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering

Bacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell lysis. The prospects for endolysins are furthered by the ability to engineer them; novel endolysins can be developed...

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Published inBiochimica et biophysica acta. Proteins and proteomics Vol. 1868; no. 1; p. 140302
Main Authors Love, Michael J., Abeysekera, Gayan S., Muscroft-Taylor, Andrew C., Billington, Craig, Dobson, Renwick C.J.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.2020
Subjects
Anti-Bacterial Agents - chemistry
Antibacterial
Bacteriophages - enzymology
Catalysis
Catalytic mechanism
Endolysin
Enzyme engineering
Hydrolases - chemistry
Protein Engineering
Protein structure
Endolysin
Antibacterial
Enzyme engineering
Protein structure
Catalytic mechanism
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Summary:Bacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell lysis. The prospects for endolysins are furthered by the ability to engineer them; novel endolysins can be developed with optimised stability, specificity, and lytic function. But the success of endolysin engineering and application requires a comprehensive understanding of the relationship between the enzymes biochemical, biophysical and bacteriolytic properties. Here, we examine their catalytic mechanisms, opportunities for developing novel endolysins, and highlight areas where a better understanding would support their long-term success as antibacterial agents.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:1570-9639
1878-1454
DOI:10.1016/j.bbapap.2019.140302