Low Frequency of Autoantibodies against Ki-67 Antigen in Japanese Patients with Systemic Autoimmune Diseases

• Published in: Journal of autoimmunity Vol. 10; no. 5; pp. 499 - 503
• Main Authors: Muro, Yoshinao, Kano, Takahisa, Sugiura, Kazumitsu, Hagiwara, Masatoshi
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 01.10.1997; Elsevier
• Subjects: autoantibodies; autoantigens; Autoantigens - blood; Autoimmune Diseases - blood; Autoimmune Diseases - immunology; Biological and medical sciences; cDNA cloning; Cloning, Molecular; DNA, Complementary - genetics; DNA, Complementary - metabolism; General aspects; HeLa Cells; Humans; Immunoblotting; Immunopathology; Ki-67; Ki-67 Antigen - immunology; Medical sciences; nucleolus; Rheumatic Diseases - blood; nucleolus; Ki-67; cDNA cloning; autoantibodies; autoantigens; Human; Cell proliferation; Complementary DNA; Immunological investigation; Systemic disease; Biological marker; Autoimmune disease; Monoclonal antibody; Japanese
• ISSN: 0896-8411; 1095-9157
• DOI: 10.1006/jaut.1997.0157

The Ki-67 antigen, which is recognized by the monoclonal antibody Ki-67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.