Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

• Published in: Acta tropica Vol. 166; pp. 139 - 154
• Main Authors: de Oliveira, Patrícia Rosa, Anholeto, Luis Adriano, Bechara, Gerváso Henrique, Camargo Mathias, Maria Izabel
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Acaricide; Acaricides - pharmacology; Animals; Control; Cytoplasm - drug effects; Cytoplasm - ultrastructure; Digestive System - drug effects; Digestive System - ultrastructure; Dinotefuran; Egg Yolk - drug effects; Egg Yolk - ultrastructure; Embryonic Germ Cells - drug effects; Embryonic Germ Cells - ultrastructure; Female; Fertility - drug effects; Guanidines - pharmacology; Neonicotinoids; Nitro Compounds - pharmacology; Organelles - drug effects; Organelles - ultrastructure; Ovary - drug effects; Ovary - ultrastructure; Rhipicephalus sanguineus; Rhipicephalus sanguineus - drug effects; Rhipicephalus sanguineus - ultrastructure; Ultrastructure; Vitellogenesis - drug effects; Dinotefuran; Control; Acaricide; Ultrastructure; Rhipicephalus sanguineus
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2016.11.017

Ultrastructure of the dinotefuran-treated semi-engorged females tick Rhipicephalus sanguineus. (A.C.) Midgut of the control group. (B. D.) Midgut of group IV. (E. G. I.) Ovary of the control group. (F. H. J. K.) Ovary of the group IV. [Display omitted] •The present study analyzed the effects of dinotefuran on the ovary and midgut cells of ticks.•The individuals revealed the action of this chemical showing alterations in the germ cells and midgut.•The alterations were mainly related to the damaged cell structures and structures related with defense mechanisms in these cells.•The effects demonstrate the progressive damages in the midgut and germ cells of R. sanguineus semi-engorged females.•The concentrations applied partially impaired the digestive processes and the vitellogenesis. The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet®) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days. The results showed alterations mainly regarding the damaged cell structures, such as yolk granules, organelles and the plasma membrane of the germ cells. In addition, structures related with defense mechanisms were found, such as vacuoles, cytoskeletal filaments, and myelin figures in the germ cells. Damages in the generative cells of the midgut, alterations in the size of digestive cells, the number of endosomes, digestive vacuoles, digestive residues, lipid drops and organelles in the cytoplasm of the digestive cells and the presence of microvilli in the plasma membrane of these cells also demonstrate the progressive damages caused by the action of dinotefuran in the midgut and germ cells of R. sanguineus semi-engorged females. The concentrations applied partially impaired the digestive processes; and, without proper nutrition, all the ectoparasite’s physiologic events are prevented from occurring, leading the individual to death. The germ cells were also damaged, and probably would not be able to advance in their development (I–V) and complete the vitellogenesis, which would affect the fertility of the female and consequently impede the formation of a new individual.