Expression of the Antimicrobial Peptide SE-33-A2P, a Modified Analog of Cathelicidin, and an Analysis of Its Properties

• Published in: Antibiotics (Basel) Vol. 13; no. 2; p. 190
• Main Authors: Gasanov, Vagif, Vorotelyak, Ekaterina, Vasiliev, Andrey
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2024
• Subjects: Amino acids; AMP; Antibiotic resistance; Antibiotics; Antimicrobial activity; Antimicrobial agents; antimicrobial peptide expression; Antimicrobial peptides; Bacteria; cathelicidin; Cell culture; Chemical synthesis; Chromatography; Drug resistance in microorganisms; E coli; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Lipids; LL-37; Peptides; Proteins; recombinant protein; SE-33; Wisconsin; LL-37; AMP; cathelicidin; recombinant protein; cholesterol; SE-33; cloning; A2P; antimicrobial peptide expression
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics13020190

In this study, we developed a method for the expression of the antimicrobial peptide SE-33-A2P in bacterial cells. The SE-33-A2P peptide consists of A2P and SE-33 peptides and is a retro analog of cathelicidin possessing antimicrobial activity against both Gram-positive and Gram-negative bacteria. Furthermore, the A2P peptide is a self-cleaving peptide. For an efficient expression of the SE-33-A2P peptide, a gene encoding several repetitive sequences of the SE-33 peptide separated by A2P sequences was created. The gene was cloned into a plasmid, with which cells were transformed. An induction of the product expression was carried out by IPTG after the cell culture gained high density. The inducible expression product, due to the properties of the A2P peptide, was cleaved in the cell into SE-33-A2P peptides. As the next step, the SE-33-A2P peptide was purified using filtration and chromatography. Its activity against both Gram-positive and Gram-negative bacteria, including antibiotic-resistant bacteria, was proved. The developed approach for obtaining a prokaryotic system for the expression of a highly active antimicrobial peptide expands the opportunities for producing antimicrobial peptides via industrial methods.