Effect of C/N substrates for enhanced extracellular polymeric substances (EPS) production and Poly Cyclic Aromatic Hydrocarbons (PAHs) degradation

• Published in: Environmental pollution (1987) Vol. 275; p. 116035
• Main Authors: Premnath, N., Mohanrasu, K., Guru Raj Rao, R., Dinesh, G.H., Siva Prakash, G., Pugazhendhi, Arivalagan, Jeyakanthan, J., Govarthanan, Muthusamy, Kumar, Ponnuchamy, Arun, A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.04.2021
• Subjects: Biodegradation; Biodegradation, Environmental; Biofilm; EPS; Extracellular Polymeric Substance Matrix; Hydrocarbons, Aromatic; Klebsiella pneumoniae; Marine bacteria; Nitrogen; PAHs; Polycyclic Aromatic Hydrocarbons; Biodegradation; Biofilm; EPS; PAHs; Klebsiella pneumoniae; Marine bacteria
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2020.116035

Extracellular Polymeric Substances (EPS) influenced Poly Cyclic Aromatic Hydrocarbons (PAHs) degrading Klebsiella pneumoniae was isolated from the marine environment. To increase the EPS production by Klebsiella pneumoniae, several physicochemical parameters were tweaked such as different carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch, and sucrose at 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at 2 g/L), different pH, carbon/nitrogen ratio, temperature, and salt concentration were examined. Maximum EPS growth and biodegradation of Anthracene (74.31%), Acenaphthene (67.28%), Fluorene (62.48%), Naphthalene (57.84%), and mixed PAHs (55.85%) were obtained using optimized conditions such as glucose (10 g/L) as carbon source, potassium nitrate (2 g/L) as the nitrogen source at pH 8, growth temperature of 37 °C, 3% NaCl concentration and 72 h incubation period. The Klebsiella pneumoniae biofilm architecture was studied by confocal laser scanning microscopy (CLSM) and scanning electron microscope (SEM). The present study demonstrates the EPS influenced PAHs degradation of Klebsiella pneumoniae. [Display omitted] •Biofilm mediated PAHs degrading Klebsiella pneumonia isolated from marine region.•Biofilm mediated PAHs biodegrading optimization studies were done.•Maximum biofilm mediated PAHs degradation was obtained at 72 h.•Biofilm architecture along with PAHs was confirmed by SEM and CLSM studies.