Immunochromatographic assay for melamine based on luminescent quantum dot beads as signaling probes
To screen and detect the harmful substance melamine (MEL), a quantum-dot-bead-based immunochromatographic assay (QB-ICA) was formulated. After optimization, calibration was performed within the linear range from 0.06 to 0.28 ng mL −1 , with limit of detection (LOD) of 0.04 ng mL −1 . The LOD was 35...
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Published in | RSC advances Vol. 1; no. 6; pp. 337 - 3313 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
20.01.2020
The Royal Society of Chemistry |
Subjects | |
Online Access | Get full text |
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Summary: | To screen and detect the harmful substance melamine (MEL), a quantum-dot-bead-based immunochromatographic assay (QB-ICA) was formulated. After optimization, calibration was performed within the linear range from 0.06 to 0.28 ng mL
−1
, with limit of detection (LOD) of 0.04 ng mL
−1
. The LOD was 35 times lower than that of ICA that used colloidal gold nanoparticles (LOD = 1.4 ng mL
−1
) and 40 times lower than that of the assay based on quantum dots (LOD = 1.6 ng mL
−1
). In the detection of MEL in spiked pure milk using the proposed QB-ICA strategy, the LOD (LOD = 0.19 ng mL
−1
) of the samples with the proposed pretreatment was 18.4 times lower than those of the samples without pretreatment (LOD = 3.5 ng mL
−1
). The performance and practicability of the proposed QB-ICA system was validated; the obtained results reveal that QB-ICA is comparable with the conventional enzyme-linked immunosorbent assay (ELISA) method, but with enhanced applicability. Given its high sensitivity and practicability, the QB-ICA strategy could become a worthwhile alternative for the rapid, sensitive, and quantitative onsite detection of harmful substances, facilitating food safety monitoring.
An immunochromatographic assay using quantum dot beads as a label was established for melamine detection in milk with fast and effective pretreatment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 The authors contribute equally to the work. |
ISSN: | 2046-2069 2046-2069 |
DOI: | 10.1039/c9ra08350b |