Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin

• Published in: Nature biotechnology Vol. 18; no. 10; pp. 1101 - 1104
• Main Authors: Datta, Swapan Kumar, Tu, Jumin, Zhang, Guoan, Datta, Karabi, Xu, Caiguo, He, Yuqing, Zhang, Qifa, Khush, Gurdev Singh
• Format: Journal Article
• Language: English
• Published: New York, NY Nature 01.10.2000; Nature Publishing Group
• Subjects: Actins - genetics; Animals; Bacillus thuringiensis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Toxins; Biological and medical sciences; Biological control; China; Control; CryIA(b) toxin; CryIA(c) toxin; Endotoxins - genetics; Endotoxins - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genetic Engineering; Hemolysin Proteins; Homozygote; Hybridization, Genetic; Insecticides - metabolism; Lepidoptera - physiology; Oryza - genetics; Oryza - growth & development; Oryza - parasitology; Oryza sativa; Pest Control, Biological; Phenotype; Phytopathology. Animal pests. Plant and forest protection; Plants, Genetically Modified; Promoter Regions, Genetic - genetics; Protozoa. Invertebrates; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Transgenes - genetics; China; Performance evaluation; Monocotyledones; Bacillus thuringiensis; Biological control; Hybrid; Bacillaceae; Gene expression; Field experiment; Cereal crop; Toxin; Oryza sativa; Bacillales; Gramineae; Angiospermae; Bacteria; Spermatophyta; Transgenic plant; Recombinant protein; Parasporal body; Microbial insecticide
• ISSN: 1087-0156; 1546-1696
• DOI: 10.1038/80310

Here we describe development of transgenic elite rice lines expressing a Bt fusion gene derived from cryIA(b) and cryIA(c) under the control of rice actinI promoter. The lines used in the study were indica CMS restorer line of Minghui 63 and its derived hybrid rice Shanyou 63. The level of Bt fusion protein CryIA(b)/CryIA(c) detected in Minghui 63 (T51-1) plants was 20 ng/mg soluble protein. The Bt Shanyou 63 was field-tested in natural and repeated heavy manual infestation of two lepidopteran insects, leaffolder and yellow stem borer. The transgenic hybrid plants showed high protection against both insect pests without reduced yield.