Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand: Evidence for bovine-to-human zoonotic transmission

• Published in: Infection, genetics and evolution Vol. 63; pp. 43 - 57
• Main Authors: Tacharoenmuang, Ratana, Komoto, Satoshi, Guntapong, Ratigorn, Ide, Tomihiko, Singchai, Phakapun, Upachai, Sompong, Fukuda, Saori, Yoshida, Yumika, Murata, Takayuki, Yoshikawa, Tetsushi, Ruchusatsawat, Kriangsak, Motomura, Kazushi, Takeda, Naokazu, Sangkitporn, Somchai, Taniguchi, Koki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2018
• Subjects: Bovine origin; G10P strains; Group A rotaviruses; Thailand; Whole genomic analysis; Zoonotic transmission; Bovine origin; G10P strains; Zoonotic transmission; Thailand; Whole genomic analysis; Group A rotaviruses
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2018.05.009

An unusual rotavirus strain, DB2015-066 with the G10P[14] genotype (RVA/Human-wt/THA/DB2015-066/2015/G10P[14]), was detected in a stool sample from a child hospitalized with acute gastroenteritis in Thailand. Here, we sequenced and characterized the full-genome of the strain DB2015-066. On whole genomic analysis, strain DB2015-066 was shown to have a unique genotype constellation: G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The backbone genes of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) are commonly found in rotavirus strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain DB2015-066 could be of artiodactyl (likely bovine) origin. Thus, strain DB2015-066 appeared to be derived from through zoonotic transmission of a bovine rotavirus strain. Of note, the VP7 gene of strain DB2015-066 was located in G10 lineage-6 together with ones of bovine and bovine-like rotavirus strains, away from the clusters comprising other G10P[14] strains in G10 lineage-2/4/5/9, suggesting the occurrence of independent bovine-to-human interspecies transmission events. Our observations provide important insights into the origins of rare G10P[14] strains, and into dynamic interactions between artiodactyl and human rotavirus strains. •Whole genomic analysis of a rare human G10P[14] strain isolated in Thailand.•Each of the 11 genes of strain DB2015–066 appeared to be of artiodactyl origin.•Strain DB2015–066 was assumed to have been derived through interspecies transmission.•Evidence of bovine-to-human interspecies transmission of RVA strains.