Cardiaprotective effect of crocetin by attenuating apoptosis in isoproterenol induced myocardial infarction rat model

• Published in: Biomedicine & pharmacotherapy Vol. 93; pp. 376 - 382
• Main Authors: Zhang, Weili, Li, Yuhui, Ge, Zhiming
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.09.2017
• Subjects: Animals; Antioxidant; Apoptosis; Apoptosis - drug effects; bcl-2-Associated X Protein - metabolism; Biomarkers - metabolism; Cardiotonic Agents - pharmacology; Cardiotonic Agents - therapeutic use; Carotenoids - pharmacology; Carotenoids - therapeutic use; Caspase 3 - metabolism; Crocetin; Cytokines; Cytokines - metabolism; Disease Models, Animal; Hemodynamics - drug effects; Inflammation Mediators - metabolism; Isoproterenol; Male; Myocardial infarction; Myocardial Infarction - drug therapy; Myocardial Infarction - pathology; Myocardial Infarction - physiopathology; Myocardium - enzymology; Myocardium - metabolism; Myocardium - pathology; NF-E2-Related Factor 2 - metabolism; Oxidative Stress - drug effects; Rats, Wistar; Ventricular Function, Left - drug effects; Vitamin A - analogs & derivatives; Myocardial infarction; Cytokines; Isoproterenol; Antioxidant; Crocetin; Apoptosis
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2017.06.032

Given study evaluates the cardioprotective effect of crocetin in myocardial infracted (MI) rats. MI was produced by administering isoproterenol (90mg/kg/day, i.p.) in rats for two consecutive days. all the animals were divided in to four groups such as control group receives only saline; MI group which receives only isoproterenol and crocetin treated group which receives crocetin (50, 100 and 200mg/kg/day, p.o.) for the duration of 15 days. At the end of dosing left ventricular functions was assessed to estimate its effect on cardiac functions. Catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), creatine kinase (CK-MB), lactate dehydrogenase (LDH) and inflammatory cytokines were determined in the cardiac tissue homogenate. Histopathology study was also carried out using hematoxylin and eosin staining. Immunohistochemistry was done for the estimation of Caspase-3, Bcl-2, Bax and Nrf-2 level in the myocardial tissues of MI rats. Result of the study suggested that GSH, CAT, CK-MB, and LDH were (p<0.01) increased in the tissue homogenate of crocetin treated group than MI group. However crocetin significantly (p<0.01) decreases the level of MDA and activity of SOD in the tissue homogenate than MI group. It was observed that treatment with crocetin attenuates the level of inflammatory cytokines in the myocardial tissues of MI rats. Moreover level of caspase-3, Bax and Nrf-2 significantly reduced and Bcl-2 enhanced in the myocardial tissues of MI rats than MI group. The altered cellular architecture of heart tissue sections in the myocardial infracted rats were reversed by administration of crocetin treatment. Taking all these data together, it may be suggested that the crocetin act as a possible protective agent in myocardial infarction by decreasing oxidative stress and inflammatory cytokines and thereby attenuates the apoptosis of myocardial cells.