site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

• Published in: Nucleic acids research Vol. 16; no. 20; pp. 9477 - 9487
• Main Authors: Xia, Y, Morgan, R, Schildkraut, I, Van Etten, J.L
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25.10.1988
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chlorella; Chlorella - enzymology; Chlorella - genetics; Chlorella - metabolism; Chlorophycota; DNA; DNA, Single-Stranded; DNA, Viral - genetics; DNA, Viral - isolation & purification; DNA, Viral - metabolism; DNA-Cytosine Methylases - antagonists & inhibitors; DNA-Cytosine Methylases - biosynthesis; DNA-Cytosine Methylases - isolation & purification; enzyme activity; Enzyme Induction; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Hydrolases; infection; Methylation; nucleotide sequences; plant viruses; Plant Viruses - enzymology; Plant Viruses - genetics; restriction endonucleases; Sequence specificity; Chlorella; Purification; Enzyme; Algae; Chlorophycophyta; Site specificity; Virus; Enzymatic activity; Single strand break; DNA; Restriction endonuclease; Oligonucleotide; Thallophyta
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/16.20.9477

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.