Glutathione biosynthesis plays an important role in microcystin-LR depuration in lettuce and spinach

• Published in: Environmental pollution (1987) Vol. 253; pp. 599 - 605
• Main Authors: Cao, Qing, Liu, Weijing, Jiang, Weili, Shu, Xiubo, Xie, Liqiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2019
• Subjects: Animals; Bioaccumulation; Depuration; Glutathione - biosynthesis; Glutathione Reductase; Glutathione Transferase; Humans; Lactuca - physiology; Marine Toxins; Microcystin-LR; Microcystins - metabolism; Spinacia oleracea - physiology; Vegetables; Vegetables; Bioaccumulation; Depuration; Glutathione biosynthesis; Microcystin-LR
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2019.07.064

Irrigation of crop plants with microcystins (MCs) contaminated water could be a threat to human health via bioaccumulation. Despite the fact MCs bioaccumulation in crop plants is well documented, MCs depuration, as well as the mechanism involved remains unclear. The objectives of the present study were to investigate the bioaccumulation and depuration of microcystin-LR (MC-LR) in lettuce (Lactuca sativa L.) and spinach (Spinacia oleracea L.), as well as to explore the role of glutathione (GSH) biosynthesis in MC-LR depuration. The tested plants were irrigated with deionized water containing 10 μg L−1 MC-LR for 12 days (bioaccumulation), and subsequently, with either deionized water only or deionized water containing 0.5 mM buthionine sulfoximine (BSO, a specific inhibitor of GSH biosynthesis) for 12 days (depuration). After bioaccumulation period, highest concentrations of MC-LR found in lettuce and spinach were 114.4 and 138.5 μg kg−1 dry weight (DW) respectively. Depuration rates of MC-LR in lettuce and spinach were 9.5 and 8.1 μg kg−1 DW d−1, which deceased to 3.7 and 4.6 μg kg−1 DW d−1 in treatments with BSO application. GSH content in both lettuce and spinach were not significantly affected during depuration without BSO; whereas after treatment with BSO, GSH content significantly decreased by 36.0% and 24.7% in lettuce and spinach on 15 d, and the decrease remained on 18 d and 21 d in lettuce. Moreover, during the bioaccumulation period, activities of glutathione reductase (GR) and glutathione S-transferase (GST) were enhanced in both plants. Our results suggested that GSH biosynthesis played an important role in MC-LR depuration in the tested plants. Concerning human health risk, most of the estimated daily intake (EDI) values during the bioaccumulation period exceeded the tolerable daily intake (TDI) guideline. However, the risk could be alleviated by irrigating with MCs-free water for a certain amount of time before harvest. [Display omitted] •A time-dependent bioaccumulation of microcystin-LR was observed in both plants.•Spinach bioaccumulated more microcystin-LR than lettuce.•Glutathione plays an important role in microcystin-LR depuration.•Irrigation with microcystin-free water could alleviate the risk of microcystins. GSH biosynthesis is important for the depuration of MC-LR in crop plants, which could alleviate the potential threat to human health.