Discovery of pentaene polyols by the activation of an enediyne gene cluster: biosynthetic implications for 9-membered enediyne core structures
The identification and characterization of enediyne polyketide synthases (PKSEs) revealed that PKSE-bound polyene is a common intermediate, while its subsequent tailoring steps to enediyne cores remain obscure. Herein, we report pentaene polyols 5-7 and cinnamic acid derivatives 8 and 9 biosynthesiz...
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Published in | Chemical science (Cambridge) Vol. 13; no. 45; pp. 13475 - 13481 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
CAMBRIDGE
Royal Soc Chemistry
23.11.2022
Royal Society of Chemistry The Royal Society of Chemistry |
Subjects | |
Online Access | Get full text |
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Summary: | The identification and characterization of enediyne polyketide synthases (PKSEs) revealed that PKSE-bound polyene is a common intermediate, while its subsequent tailoring steps to enediyne cores remain obscure. Herein, we report pentaene polyols
5-7
and cinnamic acid derivatives
8
and
9
biosynthesized from an activated enediyne biosynthetic gene cluster in
Streptomyces
sp. CB02130. The C-1027
pksE
could partially complement production of these polyene polyols in a CB02130 mutant where the native
pksE
is inactivated. The yields of
5-7
were improved by increasing the cellular pool of
l
-Phe through either gene inactivation of a prephenate dehydrogenase WlsPDH or supplementation of
l
-Phe. A flexible ammonia lyase WlsC4 is responsible for biosynthesis of
8
and
9
from
l
-Phe. The co-localization of
wls
PDH and
PKSE
gene cassette supports their close evolutionary relationships and an enediyne genome mining strategy using WlsPDH. These findings not only provide a facile approach to activate silent enediyne BGCs, but suggest that a polyene epoxide intermediate may be formed for construction of 9-membered enediyne macrocycles.
Production of three new pentaene polyols
5-7
by an activated enediyne gene cluster and their biosynthetic study suggest the presence of a polyene epoxide intermediate during the early steps of 9-membered enediyne core formation. |
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Bibliography: | https://doi.org/10.1039/d2sc04379c Electronic supplementary information (ESI) available: Full experimental details and procedures, Fig. S1-S61; Tables S1-S36. See DOI ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2041-6520 2041-6539 |
DOI: | 10.1039/d2sc04379c |