Quantitative analysis of proliferation and cell cycle length during development of the rat retina

The rat retina has been a useful model system for the study of the development of the central nervous system (CNS). In order to facilitate future studies on the mechanisms that control retinal growth, we have quantified the proliferation of retinal cells and the length of the cell cycle throughout d...

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Bibliographic Details
Published inDevelopmental dynamics Vol. 205; no. 3; pp. 293 - 307
Main Authors Alexiades, Macrene R., Cepko, Constance
Format Journal Article
LanguageEnglish
Published New York Wiley‐Liss, Inc 01.03.1996
Subjects
Animals
Bromodeoxyuridine - pharmacology
Cell Count
Cell Cycle
Cell Division
DNA
Isotope Labeling
Mitosis
Proliferation
Rat
Rats
Rats, Sprague-Dawley
Retina - cytology
Retina - embryology
Retina - growth & development
Retinal development
S Phase
Time Factors
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Summary:The rat retina has been a useful model system for the study of the development of the central nervous system (CNS). In order to facilitate future studies on the mechanisms that control retinal growth, we have quantified the proliferation of retinal cells and the length of the cell cycle throughout development. For each day during development, the number of mitotic and postmitotic cells per retina, the proportion of cycling cells, S phase length, and cell cycle length were determined through quantification of cell numbers and 3H‐thymidine labeling. As retinal development proceeds, the proportion of cycling cells decreases, and cell cycle length increases, in part due to an increase in S phase length. © 1996 Wiley‐Liss, Inc.
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ISSN:1058-8388
1097-0177
DOI:10.1002/(SICI)1097-0177(199603)205:3<293::AID-AJA9>3.0.CO;2-D