Multiplication stimulating activity (MSA) from the BRL 3A rat liver cell line: relation to human somatomedins and insulin

• Published in: Journal of supramolecular structure and cellular biochemistry Vol. 15; no. 3; p. 253
• Main Authors: Rechler, M M, Nissley, S P, King, G L, Moses, A C, Van Obberghen-Schilling, E E, Romanus, J A, Knight, A B, Short, P A, White, R M
• Format: Journal Article
• Language: English
• Published: United States 1981
• Subjects: Adipose Tissue - drug effects; Animals; Binding, Competitive; Biological Assay; Cell Line; Humans; Insulin - pharmacology; Insulin-Like Growth Factor II; Kinetics; Liver; Molecular Weight; Peptides - isolation & purification; Peptides - metabolism; Peptides - pharmacology; Rats; Receptors, Cell Surface - metabolism; Receptors, Somatomedin; Somatomedins - pharmacology
• ISSN: 0275-3723
• DOI: 10.1002/jsscb.1981.380150305

The properties of multiplication stimulating activity (MSA), an insulin-like growth factor (somatomedin) purified from culture medium conditioned by the BRL 3A rat liver cell line are summarized. The relationship of MSA to somatomedins purified from human and rat plasma are considered. MSA appears to be the predominant somatomedin in fetal rat serum, but a minor component of adult rat somatomedin. In vitro biological effects of MSA and insulin in adipocytes, fibroblasts and chondrocytes are examined to determine whether they are mediated by insulin receptors or insulin-like growth factor receptors. The possible relationship of a primary defect of insulin receptors observed in fibroblasts from a patient with the rare genetic disorder, leprechaunism, to intrauterine growth retardation is discussed.