Isolation of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from drinking water reservoirs in Hong Kong

• Published in: Journal of applied microbiology Vol. 103; no. 3; pp. 507 - 515
• Main Authors: Lau, S.K.P., Woo, P.C.Y., Fan, R.Y.Y., Ma, S.S.L., Hui, W.‐T., Au, S.‐Y., Chan, L.‐L., Chan, J.Y.F., Lau, A.T.K., Leung, K.‐Y., Pun, T.C.T., She, H.H.L., Wong, C.‐Y., Wong, L.L.L., Yuen, K.‐Y.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2007; Blackwell Science
• Subjects: Animals; Bacteria; Biological and medical sciences; Carassius auratus; Colony Count, Microbial; Community-Acquired Infections - microbiology; community‐acquired gastroenteritis; Disease Reservoirs - microbiology; drinking water; Electrophoresis, Gel, Pulsed-Field - methods; Environmental Microbiology; Fishes - microbiology; Food Microbiology; Fresh Water - microbiology; Freshwater; freshwater fish; Fundamental and applied biological sciences. Psychology; Gastroenteritis - microbiology; Hong Kong; Humans; Laribacter hongkongensis; Microbiology; Neisseriaceae - genetics; Neisseriaceae - isolation & purification; Oreochromis niloticus; Phenotype; Phylogeny; reservoir; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Water Microbiology; Water Supply; Hong Kong; Asia; China; China, People's Rep., Hong Kong; Drinking water; Hong Kong; Applied microbiology; community-acquired gastroenteritis; Gastroenteritis; Fresh water; Bacteria; Isolation; Reservoir; freshwater fish; Laribacter hongkongensis
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2006.03263.x

Aims:  Freshwater fish has been found to be the reservoir of Laribacter hongkongensis, a recently discovered bacterium associated with community‐acquired gastroenteritis. However, little is known about the ecology of this bacterium in the aquatic environment. We carried out a surveillance study to investigate the presence of L. hongkongensis in water and freshwater fish from 10 drinking water reservoirs in Hong Kong. Methods and Results:  Using membrane filtration, L. hongkongensis was isolated from the waters of six reservoirs, with numbers ranging from 1 to 12 CFU l−1. Higher recovery rates were observed in summer and during days of higher water and ambient temperatures. Of 27 freshwater fish collected from the reservoirs, L. hongkongensis was recovered from the intestines of two fish, a Goldfish and a Nile tilapia. Overall, 35 different pulsed‐field gel electrophoresis patterns are found among the 59 isolates recovered from water and the two isolates from freshwater fish. Conclusions:  The present report represents the first to demonstrate the presence of L. hongkongensis in natural water environments. Significance and Impact of the Study:  Although it is unlikely that treated, drinking water is an important source of L. hongkongensis‐associated gastroenteritis, one should be aware of the possibility of other contaminated water as a source of human infection.