Contribution of germline mutations to PARK2 gene inactivation in lung adenocarcinoma
Homozygous germline mutations of the PARK2 gene are responsible for the development of early‐onset Parkinson's disease (PD). Homozygous PARK2 mutations have been also detected in lung adenocarcinoma (LADC). However, since heterozygous PARK2 germline mutations are present in a subset of non‐PD i...
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Published in | Genes chromosomes & cancer Vol. 51; no. 5; pp. 462 - 472 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.05.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Homozygous germline mutations of the PARK2 gene are responsible for the development of early‐onset Parkinson's disease (PD). Homozygous PARK2 mutations have been also detected in lung adenocarcinoma (LADC). However, since heterozygous PARK2 germline mutations are present in a subset of non‐PD individuals, the timing for the occurrence of two‐hit PARK2 mutations in LADC progression is unclear. Therefore, we comprehensively analyzed mutations, expression and copy number variations of the PARK2 gene in 267 primary LADCs together with the corresponding noncancerous lung cells and 39 LADC cell lines. Heterozygous germline exonic deletions were detected in five patients with LADC, and loss of heterozygosity including the PARK2 locus was detected in 31/267 (11.6%) LADCs. However, homozygous PARK2 inactivation was not detected in any of them, including the five patients with germline mutations. Homozygous PARK2 inactivation was detected in 6/39 (15%) cell lines, two exonic deletions, one exonic duplication, and three point mutations, while heterozygous PARK2 inactivation was detected in two cell lines (both by exonic deletions). These results strongly indicate that somatic PARK2 mutations occur rarely (or do not occur) in LADC development and that germline PARK2 mutations could contribute to LADC progression but not to LADC development. © 2012 Wiley Periodicals, Inc. |
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Bibliography: | Supported by: Ministry of Health, Labor and Welfare for the 3rd-Term Comprehensive 10-year Strategy for Cancer Control. ark:/67375/WNG-7H27WN9H-P istex:6E4B47A8EF64EF005C1AEC6F5593EA8B22771861 ArticleID:GCC21933 Supported by: Ministry of Health, Labor and Welfare for the 3rd‐Term Comprehensive 10‐year Strategy for Cancer Control. |
ISSN: | 1045-2257 1098-2264 |
DOI: | 10.1002/gcc.21933 |