Glucose-Based PD Solution, But Not Icodextrin-Based PD Solution, Induces Plasminogen Activator Inhibitor-1 and Tissue-Type Plasminogen Activator in Human Peritoneal Mesothelial Cells via ERK1/2

• Published in: Therapeutic apheresis and dialysis Vol. 11; no. 2; pp. 94 - 100
• Main Authors: Katsutani, Masahira, Ito, Takafumi, Masaki, Takao, Kohno, Nobuoki, Yorioka, Noriaki
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.04.2007
• Subjects: Cells, Cultured; Dialysis Solutions - chemistry; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelium - drug effects; Epithelium - metabolism; ERK1/2; Flavonoids - adverse effects; Glucans - adverse effects; Glucans - chemistry; Glucose - adverse effects; Glucose - chemistry; Glucose - pharmacology; Human peritoneal mesothelial cell; Humans; Icodextrin; Mitogen-Activated Protein Kinase 3 - metabolism; PAI-1; PD solution; Peritoneal Dialysis; Peritoneum - cytology; Peritoneum - metabolism; Plasminogen Activator Inhibitor 1 - biosynthesis; t-PA; Tissue Plasminogen Activator - biosynthesis
• ISSN: 1744-9979; 1744-9987
• DOI: 10.1111/j.1744-9987.2007.00423.x

:  Peritoneal dialysis (PD) solutions containing glucose are considered to cause peritoneal fibrosis. Plasminogen activator inhibitor‐1 (PAI‐1) and tissue‐type plasminogen activator (t‐PA) participate in fibrogenesis of various organs, and human peritoneal mesothelial cells (HPMC) can produce PAI‐1 and t‐PA following glucose stimulation. Icodextrin has been widely used as an alternative osmotic agent. In this study, we investigated whether icodextrin‐based PD solution reduced the production of PAI‐1 and t‐PA by HPMC. We also examined the involvement of extracellular signal‐regulated kinase 1/2 (ERK1/2). Glucose‐based PD solutions increased the production of PAI‐1 and t‐PA by HPMC, whereas icodextrin‐based PD solution exerted lesser effects. Glucose‐based PD solutions activated ERK1/2, and PD98059 inhibited the production of PAI‐1 and t‐PA—responses not observed with icodextrin‐based PD solution. In conclusion, glucose‐based PD solutions, unlike icodextrin‐based PD solution, induce overproduction of PAI‐1 and t‐PA via the ERK1/2 pathway.