High-performance liquid chromatographic analysis of angiotensin II receptor antagonist valsartan using a liquid extraction method

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 766; no. 2; pp. 345 - 349
• Main Authors: Daneshtalab, Noriko, Lewanczuk, Richard Z, Jamali, Fakhreddin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.01.2002
• Subjects: Angiotensin II - metabolism; Angiotensin Receptor Antagonists; Antihypertensive Agents - blood; Antihypertensive Agents - pharmacokinetics; Chromatography, High Pressure Liquid - methods; Humans; Receptors, Angiotensin - metabolism; Reference Standards; Reproducibility of Results; Spectrometry, Fluorescence; Tetrazoles - blood; Tetrazoles - pharmacokinetics; Valine - analogs & derivatives; Valine - blood; Valine - pharmacokinetics; Valsartan; Valsartan
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/S0378-4347(01)00507-2

Purpose: To develop an easy assay for the quantitation of the angiotensin II receptor antagonist valsartan in human plasma using a liquid extraction procedure. Method: The method involves acid extraction from 1 ml human plasma with methyl- tert.-butyl ether followed by back-extraction into a basic medium. An isocratic HPLC equipped with reverse phase column and a fluorescence detector was used at room temperature. Results: The response to 10–2000 ng/ml valsartan was linear. In plasma of three human subjects given 160 mg valsartan orally, concentrations of 25–1540 ng/ml were observed. Conclusion: This convenient method is suitable for pharmacokinetic studies of valsartan.