Moderate calorie restriction attenuates age‑associated alterations and improves cardiac function by increasing SIRT1 and SIRT3 expression

• Published in: Molecular medicine reports Vol. 18; no. 4; pp. 4087 - 4094
• Main Authors: Yu, Wei, Qin, Jinjin, Chen, Chunjuan, Fu, Yucai, Wang, Wei
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.10.2018; Spandidos Publications UK Ltd
• Subjects: Age; Aging - metabolism; Animals; Autophagy; Blood pressure; Body Weight; Caloric Restriction; Cardiac function; Cardiomyocytes; Cardiovascular disease; Disease age factors; Female; Gene expression; Health aspects; Heart; Heart - physiology; Heart Function Tests; Heart rate; Heart Ventricles - anatomy & histology; Heart Ventricles - metabolism; High fat diet; Immunoblotting; Insulin; Intra-Abdominal Fat - metabolism; Laboratory animals; Life span; Lipoproteins, LDL - metabolism; Low density lipoprotein; Myocardium; Nutrient deficiency; Obesity; Organ Size; Physiological aspects; Proteins; Rats, Sprague-Dawley; Reducing diets; Rodents; Signaling peptides and proteins; SIRT1 protein; Sirtuin 1 - metabolism; Sirtuin 3 - metabolism; Sirtuins; Studies; Triglycerides - metabolism; Ventricle; Ventricular Function; United States > US
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2018.9390

Calorie restriction (CR) extends the lifespan of mammals and improves cardiac function by attenuation of age‑associated alterations. Sirtuins (SIRT) are involved in these mechanisms, however, the extent to which CR affects cardiac function and sirtuin expression remains unknown. Therefore, the present study aimed to determine to what extent CR affects cardiac function and sirtuin expression. A total of 60 female Sprague‑Dawley rats were randomly divided into four groups, including normal control (NC), 25% calorie restriction (25% CR), 45% calorie restriction (45% CR) and high‑fat diet (HF). The groups were maintained on these specific regimens for 2 months. CR rats were observed to have significantly lower body weight, heart weight, and left ventricle mass index compared with NC and HF rats. Visceral fat, triglyceride, and low density lipoprotein levels were significantly decreased in CR rats. Compared with the 25% CR group, the 45% CR group heart function decreased. The heart rate, left ventricular systolic pressure, +dp/dt and ‑dp/dt of the 45% CR rats decreased, whereas the left ventricular end‑diastolic pressure increased. To explore the molecular mechanism of CR on cardiac function, immunoblotting was used to detect the protein expression of SIRT1 and SIRT3. The 25% CR diet increased the expression of SIRT1 and SIRT3 in myocardium, whereas the 45% CR and HF diets resulted in a decrease in SIRT1 and SIRT3 expression. Moderate calorie restriction (25% CR) improves cardiac function by attenuation of age‑associated alterations in rats. SIRT1 and SIRT3 are associated with these effects.