Striatal Acetylcholine–Dopamine Imbalance in Parkinson Disease: In Vivo Neuroimaging Study with Dual-Tracer PET and Dopaminergic PET–Informed Correlational Tractography

• Published in: Journal of Nuclear Medicine Vol. 63; no. 3; pp. 438 - 445
• Main Authors: Sanchez-Catasus, Carlos A., Bohnen, Nicolaas I., D’Cruz, Nicholas, Müller, Martijn L.T.M.
• Format: Journal Article
• Language: English
• Published: United States Society of Nuclear Medicine 01.03.2022
• Subjects: Acetylcholine; Animal models; Animals; Anisotropy; Cerebral hemispheres; Cholinergics; Clinical Investigation; Clusters; Dopamine; Dopamine receptors; Female; Fluorine isotopes; Humans; In vivo methods and tests; Integrity; Medical imaging; Movement disorders; Neostriatum; Neurodegenerative diseases; Neuroimaging; Parkinson Disease - complications; Parkinson Disease - diagnostic imaging; Parkinson's disease; Positron emission; Positron emission tomography; Positron-Emission Tomography - methods; Substantia alba; Tomography; Parkinson disease; dopaminergic nigrostriatal connectivity; acetylcholine–dopamine imbalance; 18F-FEOBV PET; 11C-DTBZ PET
• ISSN: 0161-5505; 1535-5667; 2159-662X; 1535-5667
• DOI: 10.2967/jnumed.121.261939

Previous studies of animal models of Parkinson disease (PD) suggest an imbalance between striatal acetylcholine and dopamine, although other studies have questioned this. To our knowledge, there are no previous in vivo neuroimaging studies examining striatal acetylcholine-dopamine imbalance in PD patients. Using cholinergic and dopaminergic PET ( F-fluoroethoxybenzovesamicol [ F-FEOBV] and C-dihydrotetrabenazine [ C-DTBZ], respectively) and correlational tractography, our aim was to investigate the acetylcholine-dopamine interaction at 2 levels of dopaminergic loss in PD subjects: integrity loss of the nigrostriatal dopaminergic white matter tract and loss at the presynaptic-terminal level. The study involved 45 subjects with mild to moderate PD (36 men, 9 women; mean age, 66.3 ± 6.3 y, disease duration, 5.8 ± 3.6 y; Hoehn and Yahr stage, 2.2 ± 0.6) and 15 control subjects (9 men, 6 women; mean age, 69.1 ± 8.6 y). PET imaging was performed using standard protocols. We first estimated the integrity of the dopaminergic nigrostriatal white matter tracts in PD subjects by incorporating molecular information from striatal C-DTBZ PET into the fiber tracking process using correlational tractography (based on quantitative anisotropy [QA], a measure of tract integrity). Subsequently, we used voxel-based correlation to test the association of the mean QA of the nigrostriatal tract of each cerebral hemisphere with the striatal F-FEOBV distribution volume ratio (DVR) in PD subjects. The same analysis was performed for C-DTBZ DVR in 12 striatal subregions (presynaptic-terminal level). Unlike C-DTBZ DVR in striatal subregions, the mean QA of the nigrostriatal tract of the most affected hemisphere showed a negative correlation with a striatal cluster of F-FEOBV DVR in PD subjects (corrected = 0.039). We also found that the mean F-FEOBV DVR within this cluster was higher in the PD group than in the control group ( = 0.01). Cross-validation analyses confirmed these findings. We also found an increase in bradykinesia ratings associated with increased acetylcholine-dopamine imbalance in the most affected hemisphere ( = 0.41, = 0.006). Our results provide evidence for the existence of striatal acetylcholine-dopamine imbalance in early PD and may provide an avenue for testing in vivo effects of therapeutic strategies aimed at restoring striatal acetylcholine-dopamine balance in PD.