The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex

• Published in: Genes & development Vol. 10; no. 13; pp. 1699 - 1708
• Main Authors: Colot, H V, Stutz, F, Rosbash, M
• Format: Journal Article
• Language: English
• Published: United States 01.07.1996
• Subjects: Amino Acid Sequence; Cloning, Molecular; Copper - pharmacology; Drug Resistance, Microbial; Genes, Fungal - genetics; Genes, Reporter; Glutathione Transferase - genetics; Introns - genetics; Models, Genetic; Molecular Sequence Data; Nuclear Cap-Binding Protein Complex; Phosphoproteins; Recombinant Fusion Proteins - metabolism; Ribonucleoprotein, U1 Small Nuclear - metabolism; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; Ribonucleoproteins - physiology; RNA Cap-Binding Proteins; RNA Caps - metabolism; RNA Splicing - physiology; RNA, Fungal - analysis; RNA, Messenger - analysis; RNA, Small Nuclear - genetics; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; RNA-Binding Proteins - physiology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Sequence Analysis, DNA; Sequence Homology, Amino Acid
• ISSN: 0890-9369; 1549-5477
• DOI: 10.1101/gad.10.13.1699

The mechanism by which pre-mRNAs are initially recognized by the splicing machinery is not well understood. In the yeast system, commitment complexes are the earliest identified splicing complexes. They contain pre-mRNA, U1 snRNP, and the splicing factor Mud2p and probably correspond to the mammalian E complexes, which contain pre-mRNA, U1 snRNP, and the splicing factor U2AF. To identify other yeast commitment complex components, we have characterized mutant strains that are synthetic lethal with viable U1 snRNA mutations. We report here that MUD13 is a nonessential gene that encodes the yeast homolog of CBP20, the small subunit of the vertebrate nuclear cap-binding complex (CBC). Characterization of splicing in the delta-MUD13 strain and extract indicates that Mud13p is a yeast splicing factor and is the second identified non-snRNP commitment complex component. The observations also suggest that CBC interacts with other commitment complex components as well as with the substrate cap. Taken together with the accompanying results for a mammalian system, our data indicate that cap-binding proteins as well as the pre-mRNA cap contribute to early steps in spliceosome assembly.