Nucleation and growth of macrofibrils in trichocyte (hard-α) keratins

• Published in: Journal of structural biology Vol. 143; no. 1; pp. 85 - 93
• Main Authors: Bruce Fraser, R.D., Rogers, George E., Parry, David A.D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2003
• Subjects: Animals; Biophysical Phenomena; Biophysics; Disulfides - chemistry; Hair structure; Intermediate filaments; Keratin; Keratins - chemistry; Microscopy, Electron; Orthocortex; Paracortex; Polymorphism, Genetic; Sulfur - chemistry; Temperature; Tyrosine - chemistry; Wool - chemistry; Wool - ultrastructure; X-Ray Diffraction; Hair structure; Orthocortex; Keratin; Paracortex; Intermediate filaments
• ISSN: 1047-8477; 1095-8657
• DOI: 10.1016/S1047-8477(03)00118-7

The intermediate filaments (IF) in trichocyte (hard-α) keratin form ordered aggregates that are infiltrated by sulfur-rich and tyrosine-rich proteins during fiber development to give a filament-matrix texture, which is stabilized in the later stages by the formation of disulfide linkages. Two polymorphic forms of macrofibril are found in the cortical cells of fine Merino wool. In the first the packing of the IF in the macrofibril is quasi-hexagonal whilst in the second the IF are packed in cylindrical sheets around a central core. In hairs the second type generally predominate. In the present contribution specific models for the mechanisms of nucleation and growth are developed for the two types of macrofibril and their applicability tested by analyzing transmission electron micrographs of wool and hair. Evidence is presented which supports the idea that sheet formation plays an important role in both types of macrofibril assembly and it is suggested that differing intersheet interactions are responsible for the differences between the ortho- and para-types. It is shown that the increase in IF tilt with radius in the ortho-type can be related to the surface lattice of the IF as determined from X-ray diffraction studies. Two possible types of intersheet interaction in the ortho-type are discussed, the first leading to an increase of around 0.5° in IF tilt per layer and the second leading to a much larger tilt of 9.4° per layer. A crude estimate based on the decrease in visibility of the IF with increasing radius in cross-section yielded a value of 0.35°–0.7°.