The regulation of OXPHOS by extramitochondrial calcium

• Published in: Biochimica et biophysica acta Vol. 1797; no. 6-7; pp. 1018 - 1027
• Main Authors: Gellerich, Frank N., Gizatullina, Zemfira, Trumbeckaite, Sonata, Nguyen, Huu P., Pallas, Thilo, Arandarcikaite, Odeta, Vielhaber, Stephan, Seppet, Enn, Striggow, Frank
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2010
• Subjects: Animals; Antiporters - metabolism; Aralar; ATP-Mg/Pi carrier; Ca2+ uniporter; Calcium - metabolism; Calcium Channels - metabolism; Disease Models, Animal; Electron Transport Complex IV - metabolism; Extramitochondrial calcium; F0F1ATPase; FAD-glycerol-3-phosphate dehydrogenase; Glutamate respiration; Glutamic Acid - metabolism; Glycerolphosphate Dehydrogenase - metabolism; Humans; Huntington Disease - genetics; Huntington Disease - metabolism; Intramitochondrial calcium; Isocitrate dehydrogenase; Mice; Mice, Transgenic; Mitochondria - metabolism; Mitochondrial Membrane Transport Proteins - metabolism; Mitochondrial Proteins - metabolism; Mitochondrial Proton-Translocating ATPases - metabolism; Models, Biological; Oxidative Phosphorylation; Oxidoreductases - metabolism; Oxygen Consumption; Permeability transition pore; Porin; Pyruvate dehydrogenase; Regulation; Transgenic Huntington rat, R6/2 mice; Voltage-Dependent Anion Channels - metabolism; α-Ketoglutarate dehydrogenase; Porin; PT; α-Ketoglutarate dehydrogenase; PDHC; GPS; α-KGDH; PTP; Intramitochondrial calcium; AOA; PDH; α-KG; F0F1ATPase; Ca2+ uniporter; Pyruvate dehydrogenase; Permeability transition pore; WT; Isocitrate dehydrogenase; Extramitochondrial calcium; State 3glu/mal; CAG; CK; GP; AK; DHAP; COX; IMS; EGTA; CsA; httexpQ; Aralar; Oxidative phosphorylation; FAD-glycerol-3-phosphate dehydrogenase; IF1; ATP-Mg/Pi carrier; Transgenic Huntington rat, R6/2 mice; VDAC; Regulation; HD; ICDH; FAD-GPDH; Glutamate respiration; MAS
• ISSN: 0005-2728; 0006-3002; 1879-2650
• DOI: 10.1016/j.bbabio.2010.02.005

Despite extensive research, the regulation of mitochondrial function is still not understood completely. Ample evidence shows that cytosolic Ca2+ has a strategic task in co-ordinating the cellular work load and the regeneration of ATP by mitochondria. Currently, the paradigmatic view is that Cacyt2+ taken up by the Ca2+ uniporter activates the matrix enzymes pyruvate dehydrogenase, α-ketoglutarate dehydrogenase and isocitrate dehydrogenase. However, we have recently found that Ca2+ regulates the glutamate-dependent state 3 respiration by the supply of glutamate to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier. Since this activation is not affected by ruthenium red, glutamate transport into mitochondria is controlled exclusively by extramitochondrial Ca2+. Therefore, this discovery shows that besides intramitochondrial also extramitochondrial Ca2+ regulates oxidative phosphorylation. This new mechanism acts as a mitochondrial “gas pedal”, supplying the OXPHOS with substrate on demand. These results are in line with recent findings of Satrustegui and Palmieri showing that aralar as part of the malate–aspartate shuttle is involved in the Ca2+-dependent transport of reducing hydrogen equivalents (from NADH) into mitochondria. This review summarises results and evidence as well as hypothetical interpretations of data supporting the view that at the surface of mitochondria different regulatory Ca2+-binding sites exist and can contribute to cellular energy homeostasis. Moreover, on the basis of our own data, we propose that these surface Ca2+-binding sites may act as targets for neurotoxic proteins such as mutated huntingtin and others. The binding of these proteins to Ca2+-binding sites can impair the regulation by Ca2+, causing energetic depression and neurodegeneration.