Blastomyces dermatitidis lysate antigens: Antibody detection in serial serum specimens from dogs with blastomycosis

• Published in: Mycopathologia (1975) Vol. 156; no. 4; pp. 289 - 294
• Main Authors: Chester, E.M, Axtell, R.C, Scalarone, G.M
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.2003; Springer Nature B.V
• Subjects: Animals; antibodies; Antibodies, Fungal - blood; Antigens, Fungal - immunology; Biological and medical sciences; Blastomyces - isolation & purification; Blastomyces dermatitidis; Blastomycoses and paracoccidioidomycosis; blastomycosis; Blastomycosis - blood; Blastomycosis - microbiology; Blastomycosis - veterinary; dog diseases; Dog Diseases - blood; Dog Diseases - microbiology; Dogs; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Fundamental and applied biological sciences. Psychology; fungal antigens; Human mycoses; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Mycology; Mycoses; serodiagnosis; Tropical mycoses; Fissipedia; Carnivora; Blastomycosis; Mycosis; Microbiology; Antibody; Mycology; Fungi; Infection; Antigen; Vertebrata; Mammalia; Serum; immunodiagnosis; Ascomycetes; Blastomyces dermatitidis; canine blastomycosis; lysate antigens; Veterinary; Detection; Dog; ELISA assay; Thallophyta; ELISA
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1023/B:MYCO.0000003575.04692.2f

Yeast phase lysate antigens prepared from different isolates of Blastomyces dermatitidis (T-58, dog-Tennessee; T-27, polar bear-Tennessee; ERC-2, dog-Wisconsin; ER-3, woodpile-Wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-ELISA, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 days post-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. Mean absorbance values observed with the four lysate antigens at the three treatment intervals ranged from the most reactive to the least reactive as follows: T-58 (0.270, 0.210, 0.136); T-27 (0.209, 0.156, 0.096); ER-3 (0.189, 0.144, 0.089) and ERC-2 (0.158, 0.129, 0.080). Even though variations in reactivity were evidenced, the lysates prepared from isolates from various geographical regions and sources were all efficacious as antigens for the immunodiagnosis of canine blastomycosis.