Principles and applications of Multidimensional Protein Identification Technology

Multidimensional chromatography coupled to tandem mass spectrometry is an emerging technique for the analysis of proteomes and is rapidly being implemented by many researchers for proteomic analysis. In this technology profile, a particular proteomic approach known as multidimensional protein identi...

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Published inExpert review of proteomics Vol. 1; no. 3; pp. 275 - 282
Main Authors Paoletti, Andrew C, Zybailov, Boris, Washburn, Michael P
Format Journal Article
LanguageEnglish
Published England Informa Healthcare 01.10.2004
Taylor & Francis
Subjects
Animals
Bacterial Proteins - chemistry
chromatography
Humans
mass spectrometry
Mass Spectrometry - methods
MudPIT
multidimensional
Proteins - chemistry
Proteins - isolation & purification
Proteome
proteomics
Proteomics - methods
quantitative
Saccharomyces cerevisiae Proteins - chemistry
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Summary:Multidimensional chromatography coupled to tandem mass spectrometry is an emerging technique for the analysis of proteomes and is rapidly being implemented by many researchers for proteomic analysis. In this technology profile, a particular proteomic approach known as multidimensional protein identification technology (MudPIT) is discussed. In MudPIT, a biphasic microcapillary column is packed with high-performance liquid chromatography grade reversed phase and strong cation exchange packing materials, loaded with a complex peptide mixture and placed in line with quaternary high-performance liquid chromatography and a tandem mass spectrometer. MudPIT has the capability to analyze highly complex proteomic mixtures such as whole proteomes, organelles and protein complexes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1478-9450
1744-8387
DOI:10.1586/14789450.1.3.275