Preparative Purification of Polyphenols from Aronia melanocarpa (Chokeberry) with Cellular Antioxidant and Antiproliferative Activity

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 1; p. 139
• Main Authors: Gao, Ningxuan, Wang, Yuehua, Jiao, Xinyao, Chou, Shurui, Li, Enhui, Li, Bin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.01.2018; MDPI
• Subjects: Acrylic Resins - chemistry; Acrylic Resins - pharmacology; Adsorption; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Antiproliferatives; Aronia melanocarpa; Berries; Blueberries; Blueberry Plants - chemistry; Blueberry Plants - metabolism; Cell Proliferation - drug effects; Cell Survival; Desorption; Elution; Ethanol; Flow velocity; Fruit - chemistry; Fruit - metabolism; Fruits; Hep G2 Cells; Hepatocytes; Humans; Kinetics; Liver; Liver cancer; Photinia - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Polyphenols - biosynthesis; Polyphenols - isolation & purification; Polystyrenes - chemistry; Polystyrenes - pharmacology; Purification; polyphenols; antioxidant; antiproliferative; Aronia melanocarpa; purification
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23010139

The aim of this study was the purification process of polyphenols from (chokeberry), and the purification parameters were optimised by adsorption and desorption tests. By comparing adsorption and desorption ability of polyphenols from chokeberry on six kinds of macroporous resin, XAD-7 resin was selected. Experiments prove that the best purification parameters of static adsorption and desorption were sample pH = 4.0 with 4 h of adsorption; and desorption solvent is 95% ethanol (pH = 7.0) with 2 h of desorption. The best dynamic parameters were 9.3 bed volume (BV) of sample loading amount at a feeding flow rate of 2 BV/h, and washing the column with 5.8 BV of water, followed by subsequent elution with an eluent volume of 5.0 mL at an elution flow rate of 2 BV/h. Next the antioxidant and antiproliferative activity of polyphenols from chokeberry, blueberries, haskap berries was studied on HepG2 human liver cancer cells. The results show that polyphenol from chokeberry has a strong antioxidant effect. Taking into account the content of polyphenols in fruit, polyphenols from chokeberry represent a very valuable natural antioxidant source with antiproliferative products.