Intracellular Destruction of Salmonellae in Genetically Resistant Mice

• Published in: Journal of medical microbiology Vol. 30; no. 1; pp. 79 - 87
• Main Authors: Lin, F. R, Hsu, H. S, Mumaw, V. R, Nakoneczna, Irene
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.1989; Society for General Microbiology
• Subjects: Animals; Bacteriology; Biological and medical sciences; Eosinophils - microbiology; Eosinophils - ultrastructure; Fundamental and applied biological sciences. Psychology; Immunity, Innate; Macrophages - microbiology; Macrophages - ultrastructure; Male; Mice; Mice, Inbred A - genetics; Mice, Inbred A - immunology; Microbiology; Microscopy, Electron; Neutrophils - microbiology; Neutrophils - ultrastructure; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phagocytosis; Salmonella typhimurium - pathogenicity; Salmonella typhimurium - ultrastructure; Virulence; Salmonella; Destruction; Leukocyte; Rodentia; Host agent relation; Vertebrata; Microorganism resistance; Mammalia; Transmission electron microscopy; Mouse; Bacteria; Intracellular; Escherichieae; Phagocytosis; Enterobacteriaceae
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/00222615-30-1-79

Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA * Correspondence should be sent to Dr H. S. Hsu. Received January 3, 1989 Revision received April 4, 1989. Accepted April 4, 1989 Virulent Salmonella typhimurium , 2000 LD50, were injected intraperito-neally into inbred male A/J mice, genetically resistant to salmonella infection. Peritoneal exudate cells were harvested between 5 and 54 h after infection and examined by electronmicroscopy. Polymorphs were seen ingesting as well as digesting the pathogens as early as 5 h after infection. Macrophages were equally active in destroying the phagocytosed organisms throughout this period. In the meantime, the proliferation of salmonellae appeared to occur extracellularly in the peritoneal cavity as evidenced by their division.