Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 8; pp. 1947 - 1952
• Main Authors: Ten, Leonid N, Jung, Hae-Min, Im, Wan-Taek, Oh, Hyun Woo, Yang, Deok-Chun, Yoo, Soon-Ae, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.2009; Society for General Microbiology
• Subjects: Aerobiosis; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Korea; Locomotion; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Panax; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Xanthomonadaceae - classification; Xanthomonadaceae - genetics; Xanthomonadaceae - isolation & purification; Xanthomonadaceae - physiology; Korea; Bacteria; New species; Description; Taxonomy; Cultivated soil
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.004945-0

1 Department of Biology and Medicinal Science, Pai Chai University, 14 Yeonja-1-Gil, Seo-Gu, Daejeon 302-735, Republic of Korea 2 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 3 Insect Resources Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea 4 Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea Correspondence Wan-Taek Im wandra{at}kaist.ac.kr A Gram-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 191 T , was isolated from a soil sample from a ginseng field in Pocheon Province, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 191 T belongs to the family Xanthomonadaceae and is related to Dokdonella fugitiva LMG 23001 T (97.8 % sequence similarity) and Dokdonella koreensis KCTC 12396 T (96.9 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-8 and the major fatty acids were iso-C 17 : 1 9 c (30.6 %), iso-C 17 : 0 (21.6 %) and iso-C 15 : 0 (13.0 %), supporting the affiliation of strain Gsoil 191 T to the genus Dokdonella . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness values between strain Gsoil 191 T and its closest phylogenetic neighbours were below 40 %. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 191 T from recognized species of the genus Dokdonella . On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 191 T represents a novel species of the genus Dokdonella , for which the name Dokdonella ginsengisoli sp. nov. is proposed. The type strain is Gsoil 191 T (=KCTC 12564 T =DSM 17954 T =CCUG 52462 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Gsoil 191 T is AB245362 . A transmission electron micrograph of a cell of strain Gsoil 191 T and two-dimensional thin-layer chromatograms of the polar lipids of the strain are available as supplementary material with the online version of this paper.