The development of a mRNA multiplex RT-PCR assay for the definitive identification of body fluids

Abstract With current methodology, DNA profiling can identify an individual from a sample of biological material but it does not reveal what body fluid or tissue source the DNA profile originated from. We have developed a multiplex PCR system using messenger RNA (mRNA) that can identify blood, saliv...

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Bibliographic Details
Published inForensic science international : genetics Vol. 4; no. 4; pp. 244 - 256
Main Authors Fleming, Rachel I, Harbison, SallyAnn
Format Journal Article
LanguageEnglish
Published Netherlands 01.07.2010
Subjects
Animals
Blood
Cats - genetics
Chickens - genetics
DNA - genetics
DNA - isolation & purification
DNA Fingerprinting
Dogs - genetics
Electrophoresis
Female
Glucose-6-Phosphatase - genetics
Glycophorin - genetics
Guinea Pigs - genetics
Histatins - genetics
Humans
Male
Matrix Metalloproteinase 11 - genetics
Mice - genetics
Pathology
Protamines - genetics
Rabbits - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
Saliva
Salivary Proteins and Peptides - genetics
Semen
Swine - genetics
Transcriptional Elongation Factors - genetics
Transglutaminases - genetics
Ubiquitin-Conjugating Enzymes - genetics
RT-PCR multiplex
Forensic DNA
mRNA profiling
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Summary:Abstract With current methodology, DNA profiling can identify an individual from a sample of biological material but it does not reveal what body fluid or tissue source the DNA profile originated from. We have developed a multiplex PCR system using messenger RNA (mRNA) that can identify blood, saliva, semen and menstrual blood in individual stains or in mixtures of body fluids. Messenger RNA transcripts specific to each type of body fluid have been identified and a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) system developed to identify these body fluids along with three housekeeping genes. This multiplex can detect semen and seminal fluid (semen without spermatozoa present). Furthermore, we have targeted the co-isolation of RNA and DNA from the same sample and, with the RT-PCR multiplex, we can determine the type of body fluid present as well as generate a DNA profile(s) from the same stain.
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ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2009.10.006