Near-complete genome sequence and seed transmission evaluation of Physalis rugose mosaic virus from southern Brazil

• Published in: Ciência rural Vol. 51; no. 4
• Main Authors: Savi, Amanda, Gorayeb, Eduardo Silva, Nascimento, Samara Campos, Faria, Caroline Bolson de, Fajardo, Thor Vinícius Martins, Nhani Junior, Antônio, Bogo, Amauri, Casa, Ricardo Trezzi, Silva, Fabio Nascimento da
• Format: Journal Article
• Language: English; Portuguese
• Published: Santa Maria Universidade Federal de Santa Maria Centro de Ciencias Rurais 01.01.2021; Universidade Federal de Santa Maria
• Subjects: AGRONOMY; characterization; Deoxyribonucleic acid; DNA; Double-stranded RNA; Dwarfism; Enzymes; Flowers & plants; Genomes; Maximum likelihood method; Next-generation sequencing; Nucleotide sequence; Nucleotides; Phylogenetics; PhyRMV; Physalis; Physalis peruviana; Seedlings; Seeds; Sobemovirus; Software; Viral infections; Viruses; Brazil; caracterização; Sobemovirus; characterization; PhyRMV
• ISSN: 0103-8478; 1678-4596; 1678-4596
• DOI: 10.1590/0103-8478cr20200702

Physalis rugose mosaic virus (PhyRMV) causes severe damage to Physalis peruviana L., affecting vegetative parameters, fruit quantity and quality. The aim of this study was to perform a molecular characterization of PhyRMV associated with P. peruviana from commercial fields in the municipality of Lages, Santa Catarina State, Southern Brazil, and to evaluate its transmission by seeds. Plants displaying mosaic, dwarfism, and leaf malformation symptoms were collected from P. peruviana. Double-stranded RNA was extracted and submitted to cDNA library synthesis and high-throughput sequencing (HTS). For the virus transmission assay, seeds from PhyRMV-infected plants were used, and viral infection in seedlings was verified using symptomatic and molecular diagnosis. PhyRMV RNA has 4162 nucleotides (nts) and a genomic organization similar to that of other sobemoviruses and shares 97% nt identity with the previously characterized PhyRMV Piracicaba isolate. Results indicated the unlikely transmission of PhyRMV by physalis seeds. RESUMO: Physalis rugose mosaic virus (PhyRMV) causa danos severos em Physalis peruviana L., afetando características vegetativas, a quantidade e qualidade de frutos. Os objetivos desse estudo consistem na caracterização molecular do PhyRMV associado a P. peruviana coletada em campos de produção em Lages, Santa Catarina, Brasil; e avaliar a transmissão do vírus por sementes. Plantas apresentando sintomas de mosaico, deformação e nanismo foram coletadas de P. peruviana. Amostras foliares dessas plantas foram utilizadas para extração de RNA de fita dupla, síntese de biblioteca de cDNA e sequenciamento de alto rendimento. No experimento de transmissão, sementes obtidas de plantas infectadas por PhyRMV foram utilizadas, e a infecção viral nas plântulas foi avaliada por inspeção visual de sintomas e diagnóstico molecular. O RNA viral apresentou 4162 nucleotideos (nts), a organização genômica foi similar à de outros sobemovírus e apresentou 97% de identidade de nucleotídeos com o isolado de Piracicaba de PhyRMV previamente caracterizado. Os resultados obtidos sugerem que é improvável a transmissão de PhyRMV por sementes de physalis.