The multifaceted capacity of Dps proteins to combat bacterial stress conditions: Detoxification of iron and hydrogen peroxide and DNA binding

• Published in: Biochimica et biophysica acta Vol. 1800; no. 8; pp. 798 - 805
• Main Authors: Chiancone, Emilia, Ceci, Pierpaolo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2010
• Subjects: Adaptation, Biological - genetics; Adaptation, Biological - physiology; Amino Acid Sequence; Animals; Bacterial Proteins - genetics; Bacterial Proteins - physiology; DNA, Bacterial - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - physiology; Dps (DNA-binding proteins from starved cells) protein; Dps–DNA interaction; Ferroxidase activity; Humans; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - pharmacokinetics; Inactivation, Metabolic - genetics; Inactivation, Metabolic - physiology; Iron - metabolism; Iron - pharmacokinetics; Microbial survival; Models, Molecular; Molecular Sequence Data; Protection under oxidative damage condition; Protein Binding; Review; Sequence Homology, Amino Acid; Stress, Physiological - genetics; Stress, Physiological - physiology; Protection under oxidative damage condition; Dps (DNA-binding proteins from starved cells) protein; Review; Ferroxidase activity; Microbial survival; Dps–DNA interaction
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2010.01.013

The widely expressed Dps proteins, so named after the DNA-binding properties of the first characterized member of the family in Escherichia coli, are considered major players in the bacterial response to stress. The review describes the distinctive features of the “ferritin-like” ferroxidation reaction, which uses hydrogen peroxide as physiological iron oxidant and therefore permits the concomitant removal of the two reactants that give rise to hydroxyl radicals via Fenton chemistry. It also illustrates the structural elements identified to date that render the interaction of some Dps proteins with DNA possible and outlines briefly the significance of Dps–DNA complex formation and of the Dps interaction with other DNA-binding proteins in relation to the organization of the nucleoid and microbial survival. Understanding in molecular terms the distinctive role of Dps proteins in bacterial resistance to general and specific stress conditions. The state of the art is that the response to oxidative and peroxide-mediated stress is mediated directly by Dps proteins via their ferritin-like activity. In contrast, the response to other stress conditions derives from the concerted interplay of diverse interactions that Dps proteins may establish with DNA and with other DNA-binding proteins.