The effects of caffeic acid phenethyl ester on tissue damage in lung after hindlimb ischemia-reperfusion

• Published in: Pharmacological research Vol. 48; no. 4; pp. 397 - 403
• Main Authors: Çalikoglu, Mukadder, Tamer, Lulufer, Sucu, Nehir, Coskun, Banu, Ercan, Bahadir, Gul, Ali, Calikoglu, Ilker, Kanik, Arzu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.10.2003
• Subjects: Animals; Bronchoalveolar Lavage - methods; Bronchoalveolar Lavage Fluid - chemistry; Bronchoalveolar Lavage Fluid - cytology; Caffeic acid phenethyl ester; Caffeic Acids - administration & dosage; Caffeic Acids - pharmacokinetics; Caffeic Acids - therapeutic use; Capillary Permeability - drug effects; Hindlimb - blood supply; Hindlimb - injuries; Hindlimb - physiopathology; Injections, Intraperitoneal; Ischemia-reperfusion; Lung; Lung - chemistry; Lung - drug effects; Lung - ultrastructure; Male; Malondialdehyde - antagonists & inhibitors; Malondialdehyde - blood; Neutrophil Infiltration - drug effects; Peroxidase - antagonists & inhibitors; Peroxidase - chemistry; Peroxidase - drug effects; Phenylethyl Alcohol - administration & dosage; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacokinetics; Phenylethyl Alcohol - therapeutic use; Proteins - antagonists & inhibitors; Proteins - chemistry; Rats; Rats, Wistar; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; Reperfusion Injury - physiopathology; Respiratory Distress Syndrome, Adult - complications; Respiratory Distress Syndrome, Adult - drug therapy; Respiratory Distress Syndrome, Adult - physiopathology; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase - chemistry; Sodium-Potassium-Exchanging ATPase - drug effects; Time Factors; Tourniquets - adverse effects; Turkey; Turkey; Ischemia-reperfusion; Caffeic acid phenethyl ester; Lung
• ISSN: 1043-6618; 1096-1186
• DOI: 10.1016/S1043-6618(03)00156-7

Aim: The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) on the lungs as a remote organ after performing hindlimb ischemia-reperfusion (I/R) and by assessing biochemical and histopathological analysis. Methods: The animals were divided into three groups: control, I/R, and I/R with CAPE. I/R period for 8 h was performed on the right hindlimb of all the anesthesied rats in I/R and CAPE with I/R group. In the CAPE with I/R group, the animals received CAPE 10 μM by intraperitoneal injection 1 h before the reperfusion. The animals in the control and I/R groups received a similar volume of saline solution by means of intraperitoneal injection. At the end of the reperfusion period, a midsternotomy was performed. Blood, bronchoalveolar lavage (BAL) and lung tissue were obtained, and were used for biochemical and histopathological examination. Results: The tissue and serum malondyaldehyde levels were significantly lower in the control ( P=0.0001 and 0.001, respectively) and in the CAPE with I/R groups ( P=0.0001 and 0.003, respectively) compared to the I/R group. Tissue Na +-K + ATPase activity in the CAPE with I/R group was significantly higher than in the I/R group ( P=0.0001). Reduced activity was found in the I/R group compared to the control group ( P=0.0001). Myeloperoxidase activity ( P=0.001) and protein concentration ( P=0.034) in BAL were significantly reduced in CAPE-treated animals when compared with the I/R group. A decreased activity and protein concentration were found in the control group compared to the I/R group ( P=0.0001 and 0.024, respectively). The lungs of the I/R group displayed intense peribronchial and perivascular leukocytic infiltration in histopathological examination compared to the CAPE with I/R group ( P<0.05). Conclusion: CAPE seems to be effective in protecting remote organ injury caused by increased oxidative stress and neutrophil accumulation that results from an I/R injury.