The effects of caffeic acid phenethyl ester on tissue damage in lung after hindlimb ischemia-reperfusion
Aim: The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) on the lungs as a remote organ after performing hindlimb ischemia-reperfusion (I/R) and by assessing biochemical and histopathological analysis. Methods: The animals were divided into three groups: contr...
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Published in | Pharmacological research Vol. 48; no. 4; pp. 397 - 403 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Ltd
01.10.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Aim: The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) on the lungs as a remote organ after performing hindlimb ischemia-reperfusion (I/R) and by assessing biochemical and histopathological analysis.
Methods: The animals were divided into three groups: control, I/R, and I/R with CAPE. I/R period for 8 h was performed on the right hindlimb of all the anesthesied rats in I/R and CAPE with I/R group. In the CAPE with I/R group, the animals received CAPE 10
μM by intraperitoneal injection 1
h before the reperfusion. The animals in the control and I/R groups received a similar volume of saline solution by means of intraperitoneal injection. At the end of the reperfusion period, a midsternotomy was performed. Blood, bronchoalveolar lavage (BAL) and lung tissue were obtained, and were used for biochemical and histopathological examination.
Results: The tissue and serum malondyaldehyde levels were significantly lower in the control (
P=0.0001 and 0.001, respectively) and in the CAPE with I/R groups (
P=0.0001 and 0.003, respectively) compared to the I/R group. Tissue Na
+-K
+ ATPase activity in the CAPE with I/R group was significantly higher than in the I/R group (
P=0.0001). Reduced activity was found in the I/R group compared to the control group (
P=0.0001). Myeloperoxidase activity (
P=0.001) and protein concentration (
P=0.034) in BAL were significantly reduced in CAPE-treated animals when compared with the I/R group. A decreased activity and protein concentration were found in the control group compared to the I/R group (
P=0.0001 and 0.024, respectively).
The lungs of the I/R group displayed intense peribronchial and perivascular leukocytic infiltration in histopathological examination compared to the CAPE with I/R group (
P<0.05).
Conclusion: CAPE seems to be effective in protecting remote organ injury caused by increased oxidative stress and neutrophil accumulation that results from an I/R injury. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1043-6618 1096-1186 |
DOI: | 10.1016/S1043-6618(03)00156-7 |