Characterization of somatic embryo attached structures in Feijoa sellowiana Berg. (Myrtaceae)

• Published in: Protoplasma Vol. 242; no. 1-4; pp. 95 - 107
• Main Authors: Correia, Sandra M, Canhoto, Jorge M
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.06.2010; Springer Vienna; Springer Nature B.V
• Subjects: Acca sellowiana; anatomy & histology; Biomedical and Life Sciences; Cell Biology; cell proliferation; cell walls; culture media; cytology; Embryo; embryogenesis; embryology; Embryonic Development; Embryos; Feijoa; Feijoa - anatomy & histology; Feijoa - cytology; Feijoa - embryology; Feijoa - ultrastructure; Life Sciences; Myrtaceae; nutrition; Original Article; Plant Sciences; plasmodesmata; Seeds; Seeds - anatomy & histology; Seeds - cytology; Seeds - ultrastructure; somatic embryogenesis; somatic embryos; starch granules; Suspensor; ultrastructure; Zoology; Myrtaceae; Suspensor; Embryo; Somatic embryogenesis; Pineapple guava
• ISSN: 0033-183X; 1615-6102; 1615-6102
• DOI: 10.1007/s00709-010-0130-z

The presence of an attached organ to somatic embryos of angiosperms connecting the embryo to the supporting tissue has been a subject of controversy. This study shows that 67% of the morphologically normal somatic embryos of Feijoa sellowiana possess this type of organ and that its formation was not affected by culture media composition. Histological and ultrastructural analysis indicated that the attached structures of somatic embryos displayed a great morphological diversity ranging from a few cells to massive and columnar structures. This contrast with the simple suspensors observed in zygotic embryos which were only formed by five cells. As well as the suspensor of zygotic embryos, somatic embryo attached structures undergo a process of degeneration in later stages of embryo development. Other characteristic shared by zygotic suspensors and somatic embryo attached structures was the presence of thick cell walls surrounding the cells. Elongated thin filaments were often associated with the structures attached to somatic embryos, whereas in other cases, tubular cells containing starch grains connected the embryo to the supporting tissue. These characteristics associated with the presence of plasmodesmata in the cells of the attached structures seem to indicate a role on embryo nutrition. However, cell proliferation in the attached structures resulting into new somatic embryos may also suggest a more complex relationship between the embryo and the structures connecting it to the supporting tissue.