Protective effect of histidine on para-nonylphenol-enhanced hydroxyl free radical generation induced by 1-methyl-4-phenylpyridinium ion (MPP +) in rat striatum

• Published in: Biochimica et biophysica acta Vol. 1568; no. 2; pp. 171 - 175
• Main Authors: Obata, Toshio, Kubota, Shunichiro, Yamanaka, Yasumitsu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.12.2001
• Subjects: 1-Methyl-4-phenylpyridinium - antagonists & inhibitors; 1-Methyl-4-phenylpyridinium ion (MPP +); Animals; Corpus Striatum - drug effects; Corpus Striatum - metabolism; Dopamine; Dopamine - analysis; Dopamine - metabolism; Free Radicals - metabolism; Histidine; Histidine - pharmacology; Hydroxybenzoates - analysis; Hydroxybenzoates - metabolism; Hydroxyl radical; Hydroxyl Radical - metabolism; Male; Microdialysis; para-Nonylphenol; Parkinson’s disease; Phenols - antagonists & inhibitors; Phenols - pharmacology; Rats; Rats, Wistar; Reserpine; Singlet oxygen; DA, dopamine; Histidine; Singlet oxygen; Dopamine; DHBA, 2,3-dihydroxybenzoic acid; 1-Methyl-4-phenylpyridinium ion (MPP +); para-Nonylphenol; MPP +, 1-methyl-4-phenylpyridinium ion; Hydroxyl radical; Parkinson’s disease
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(01)00214-8

The present study examined the antioxidant effect of histidine, a singlet oxygen ( 1O 2) scavenger, on para-nonylphenol (an environmental estrogen-like chemical)-enhanced hydroxyl radical (·OH) generation induced by 1-methyl-4-phenylpyridinium ion (MPP +) in extracellular fluid of rat striatum. Rats were anesthetized, and sodium salicylate in Ringer’s solution (0.5 nmol/μl/min) was infused through a microdialysis probe to detect the generation of ·OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Introduction of para-nonylphenol (10 μM) significantly enhanced MPP +-induced ·OH generation. Histidine (25 mM) decreased the para-nonylphenol-enhanced ·OH formation. Although the level of MPP +-induced ·OH formation trapped as DHBA after para-nonylphenol treatment increased, para-nonylphenol failed to increase either the level of dopamine and DHBA formation in the reserpinized animals. These results indicate that para-nonylphenol and MPP +-enhanced ·OH generation was based on 1O 2 production, and histidine may have a preventive effect on para-nonylphenol and MPP +-induced ·OH generation in rat striatum.